ZFIN ID: ZDB-FIG-171227-34
Mei et al., 2014 - Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms. Developmental Biology   392(2):245-55 Full text @ Dev. Biol.
Anatomical Terms:
Stage: Protruding-mouth
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 1

pk2 knockdown leads to body curvature and IPL neurogenesis defects in zebrafish. (A)–(D) Morphology of uninjected or pk2 MO injected embryos at 28 hpf. Injection of pk2 MO leads to body curvature phenotypes at different severity, from normal like to severe. (E) and (F). pk2 Expression in the retina sections. (E) Sense control; (F) anti-sense. pk2 is enriched in the ganglion cell layer and inner nuclear layer. (G)–(H′) Retinal neurogenesis defects in pk2 knockdown embryos. (G)–(H)_ Whole eye sections; (G′)–(H′) zoom-in view of boxed areas in (G)–(H). pk2 Knockdown leads to disorganization of inner plexiform layer synaptic connections, characterized by discontinuous layers. Scale bars are 30 μm in each image.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
prickle2b WT standard conditions Protruding-mouth retinal ganglion cell layer ISH
Protruding-mouth retinal inner nuclear layer ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-prickle2b standard conditions Prim-5 whole organism anterior-posterior axis decreased length, abnormal
Prim-5 whole organism increased curvature, abnormal
knu3Tg + MO3-prickle2b standard conditions Protruding-mouth neural retina development disrupted, abnormal
Protruding-mouth retinal inner plexiform layer disorganized, abnormal
Protruding-mouth retinal inner plexiform layer synapse disorganized, abnormal
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 392(2), Mei, X., Westfall, T.A., Zhang, Q., Sheffield, V.C., Bassuk, A.G., Slusarski, D.C., Functional characterization of Prickle2 and BBS7 identify overlapping phenotypes yet distinct mechanisms, 245-55, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.