ZFIN ID: ZDB-FIG-171120-8
Kenyon et al., 2016 - Expression and knockdown of zebrafish folliculin suggests requirement for embryonic brain morphogenesis. BMC Developmental Biology   16:23 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 14-19 somites

Fig. 3

Cell cycle defects in flcn morphant embryos at 18 somite stage of development. Zebrafish Fucci embryo injected with flcn sp2 mismatch control morpholino imaged in (a) Brightfield, (b) fluorescence for Geminin-Azami Green (S/G2/M phase), (c) fluorescence for Cdt1-Kusabira Orange2 and (d) fluorescence merge of Geminin-Azami Green (S/G2/M phase) and Cdt1- Kusabira Orange2 (G1). Zebrafish Fucci embryo injected with flcn sp2 morpholino imaged in (e) Brightfield, (f) fluorescence for Geminin-Azami Green (S/G2/M phase), (g) fluorescence for Cdt1-Kusabira Orange2 and (h) fluorescence merge of Geminin-Azami Green (S/G2/M phase) and Cdt1- Kusabira Orange2 (G1). i Pixel intensity of Geminin-Azami Green in embryos injected with flcn sp2 mopholino injected embryos (solid green line (MO)) when compared with flcn sp2 mismatch control morpholino injected embryos (dashed green line (MM)). Green lines show empirical log(intensity) data distributions. j Pixel intensity of Cdt1-Kusabira Orange2 in embryos injected with flcn sp2 mopholino injected embryos (solid red line (MO)) when compared with flcn sp2 mismatch control morpholino injected embryos (dashed red line (MM)). Red lines show empirical log(intensity) data distributions. Vertical, black lines indicate means, with parameter confidence distributions shown as curved black lines. Vertical, black lines indicate means, with parameter confidence distributions shown as curved black lines

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry ncv3Tg; ncv4Tg control 14-19 somites brain nucleus IFL
14-19 somites optic vesicle nucleus IFL
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites brain nucleus IFL
14-19 somites optic vesicle nucleus IFL
Venus ncv3Tg; ncv4Tg control 14-19 somites brain nucleus IFL
14-19 somites optic vesicle nucleus IFL
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites brain nucleus IFL
14-19 somites optic vesicle nucleus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites brain nucleus Venus expression decreased amount, abnormal
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites brain nucleus mCherry expression increased amount, abnormal
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites brain regulation of cell cycle decreased process quality, abnormal
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites optic vesicle nucleus Venus expression decreased amount, abnormal
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites optic vesicle nucleus mCherry expression increased amount, abnormal
ncv3Tg; ncv4Tg + MO3-flcn standard conditions 14-19 somites retina regulation of cell cycle decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.