FIGURE

Fig. 4

ID

ZDB-FIG-171023-22

Publication

Bohovych et al., 2015 - Metalloprotease OMA1 Fine-tunes Mitochondrial Bioenergetic Function and Respiratory Supercomplex Stability

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Fig. 4

Depletion of Oma1 in fish affects development.

(A,B) Representative images of larval zebrafish at 72 hours post fertilization (hpf) injected with either a standard control MO (A) or a zfoma1 MO (B). Fish injected with zfoma1 MO have smaller heads and eyes (red arrow) and defects in heart morphology (blue arrow). Bar, 500 μm. (C) Body length of control and zfoma1 morphants at 72 hpf. Average standard length was plotted ± S.E.M. (n = 6–13, **p < 0.01 by t-test). (D) Immunoblot of protein extracts from injected fish (yolk removed) at 24, 48 and 72 hpf. Expression of Oma1, Opa1 isoforms (a–f) and Cox4 (mitochondrial abundance marker) was analyzed with respective antibodies. Actin and Ponceau S staining were loading controls. Source data (full-length blots) are available online in Supplementary information.

Expression Data

Gene:	oma1
Antibodies:	Ab1-oma1 AB2-cox4
Fish:	AB AB + MO1-oma1
Knockdown Reagent:	MO1-oma1
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-oma1
Knockdown Reagent:	MO1-oma1
Observed In:	eye pericardium whole organism
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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