FIGURE

Fig. 2

ID

ZDB-FIG-171018-43

Publication

Sun et al., 2017 - Alkbh4 and Atrn Act Maternally to Regulate Zebrafish Epiboly

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Fig. 2

Morpholino knockdown of alkbh4 leads to epiboly initiation and progression delays. (A) Effectiveness of alkbh4 morpholinos. 50 pg alkbh4-5'UTR-GFP plasmid DNA was injected alone or with 10 ng std-MO, alkbh4-MO1 or alkbh4-MO2 at 1-cell stage. The injected embryos were observed for GFP expression at late gastrulation stages. (B) Morpholino knockdown of alkbh4 leads to epiboly delay in a dose dependent manner. Embryos were observed and photographed at indicated stages. Magnified observations of the embryonic marginal cell layers are shown at the last panel. (C) Epiboly initiation in (B) was measured by the percentage of embryos with yolk cell doming at 5 hpf. (D) Epiboly progression in (B) was measured by the average epiboly percentage of the embryos at 8 hpf. Ne, the number of observed embryos. *, p<0.01. Scale bars: 500 μm in (A); 100 μm in (B).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-alkbh4 TU + MO2-alkbh4
Knockdown Reagents:	MO1-alkbh4 MO2-alkbh4
Observed In:	epiboly involved in gastrulation with mouth forming second
Stage Range:	30%-epiboly to Bud

Phenotype Detail

Acknowledgments

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