ZFIN ID: ZDB-FIG-170830-15
Rasouli et al., 2017 - Regulation of cardiomyocyte behavior in zebrafish trabeculation by Neuregulin 2a signaling. Nature communications   8:15281 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. S9

Myocardial specific nrg2a overexpression leads to an increase in cardiomyocyte proliferation

(a-d) 3D views (maximum intensity z-projections) of Tg(myl7:mVenus-gmnn) (a and c) or Tg(myl7:mVenus-gmnn);Tg(myl7:nrg2a-p2a-tdTomato) (b and d) hearts at 78 (a-b) and 96 (c-d) hpf. Proliferating cardiomyocytes are labeled in green; white and yellow dashed boxes outline the ventricular and atrial chambers, respectively; V: ventricle, At: atrium; scale bars, 50 μm. (e-f) Graphs showing the average number of proliferating cardiomyocytes per heart (e) and per ventricular or atrial (f) chamber at 78 and 96 hpf; N=9 hearts; values represent means ± SEM; * P≤ 0.05,** P≤ 0.01 by Student’s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Tomato bns140Tg; ncv43Tg control Protruding-mouth atrial myocardium IFL
Protruding-mouth ventricular myocardium IFL
Day 4 atrial myocardium IFL
Day 4 ventricular myocardium IFL
Venus bns140Tg; ncv43Tg control Protruding-mouth atrial myocardium IFL
Protruding-mouth ventricular myocardium IFL
Day 4 atrial myocardium IFL
Day 4 ventricular myocardium IFL
ncv43Tg control Protruding-mouth atrial myocardium IFL
Protruding-mouth ventricular myocardium IFL
Day 4 atrial myocardium IFL
Day 4 ventricular myocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bns140Tg; ncv43Tg control Protruding-mouth atrial myocardium cardiac muscle cell proliferation increased occurrence, abnormal
Protruding-mouth ventricular myocardium cardiac muscle cell proliferation increased occurrence, abnormal
Day 4 atrial myocardium cardiac muscle cell proliferation increased occurrence, abnormal
Day 4 ventricular myocardium cardiac muscle cell proliferation increased occurrence, abnormal
Acknowledgments:
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