FIGURE

Fig. 3

ID
ZDB-FIG-170512-26
Publication
Berberoglu et al., 2017 - Satellite-like cells contribute to pax7-dependent skeletal muscle repair in adult zebrafish
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Fig. 3

Satellite-like cells in adult zebrafish skeletal muscle proliferate after mechanical injury. (A) Schematic of experimental procedure, in which mechanical injury is performed by a single needle-stick into tail skeletal muscle. The approximate position of needle-stick injury in the ventral myotome is indicated in cross-sectional view. EdU was administered (gray arrowhead) by intraperitoneal injection at each post-injury time-point, allowing for EdU incorporation into S-phase cells, and fish were sacrificed 4 h later (acute EdU labeling). Tissue was collected at 2, 3, 4, 5, 6, and 7 dpi. (B) Pax7-positive cell numbers increase at the injury site and peak at 4 days post-injury (dpi). (C) Acute EdU labeling indicates robust proliferation at the injury site at 2 and 3 dpi that declines thereafter. (D) Overlap of Pax7 and EdU at 3 dpi indicates satellite-like cell proliferation post-injury. (E) Quantification of Pax7 and EdU double positive cells at each post-injury time-point reveals a peak number of proliferating satellite-like cells at 3 dpi in a 375 µm2 area encompassing the injury site; p*<0.05; p**<0.01. (F) Quantification of the percentage of Pax7-positive cells that are EdU-positive (out of total Pax7-positive cell population) at each post-injury time-point indicates that nearly 50% of Pax7-positive cells are proliferating at 2 dpi, and ~35% are proliferating at 3 dpi. The percentage of Pax7-positive cells that are EdU-positive at later post-injury time-points (4–7 dpi) is significantly less; p*<0.05; p**<0.01. One-way ANOVA followed by Tukey's multiple comparisons test was performed for statistical analyses. Scale bar in all panels is 75 µm.

Expression Data
Antibody:
Fish:
Condition:
Anatomical Term:
Stage Range: Days 45-89 to Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage Range: Days 45-89 to Adult

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Biology, 424(2), Berberoglu, M.A., Gallagher, T.L., Morrow, Z.T., Talbot, J.C., Hromowyk, K.J., Tenente, I.M., Langenau, D.M., Amacher, S.L., Satellite-like cells contribute to pax7-dependent skeletal muscle repair in adult zebrafish, 162-180, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.