FIGURE

Fig. S4

ID
ZDB-FIG-170321-21
Publication
Zhang et al., 2017 - Left Habenula Mediates Light-Preference Behavior in Zebrafish via an Asymmetrical Visual Pathway
Other Figures
All Figure Page
Back to All Figure Page
Fig. S4

Single Cell Labeling and In Vivo Whole-cell Recording Show Contacts between RGC Axons and EmT Neurons at the AF4, and Synaptic Connections Made by EmT Projection Fibers on Hb Neurons.

(A) Sparse labeling of a single neuron in the R-EmT by transiently expressing EGFP and dense labeling of RGC axons by injecting DiD into the left eye in Tg(Gal4s1020t,UAS:mCherry) larvae. Projected confocal images show that an EGFP-expressing neuron in the EmT region contacts DiD-positive RGC axons (blue) at the AF4. Asterisk, left retina. A, anterior; L, left; P, posterior; R, right. (B) Three-dimensional representation of the case shown in (A). DiD-labeled RGC axons are in blue, an EGFP-expressing EmT neuron is in green, and mCherry-expressing thalamic neurons are in red. The EmT neuron is contacted by RGC axons at the AF4 and sends projection fibers to the ipsilateral R-Hb and then to the contralateral L-Hb via the habenular commissure (arrowhead). A, anterior; D, dorsal; L, left; P, posterior; R, right; V, ventral. (C) In vivo whole-cell recording of L-dHb neurons in response to local electrical stimulation of the ipsilateral EmT. Left, recording diagram; Middle, example traces of electrically evoked EPSCs (eEPSCs) in a L-dHb neuron before and after bath application of CNQX (50 μM). The electrical artifact (arrow) was truncated for clarification. Right, summary. Data obtained from the same neuron are connected by a dashed line. Error bars, SEM. **p < 0.01 (paired two-tailed Student’s t test).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron