Fig. S4

Cardiac parameters and vasculature defects are observed in desma^Sa5-/-, desma^ct122aGt homozygous embryos and desma^ct122aRGt homozygous embryos. ct122aRGt indicates desma^ct122aRGt homozygous embryos (related to Fig.3). Side view of a zebrafish embryo highlighting the embryonic circulatory network, where the heart and the dorsal aorta (DA) are underlined in red and the venous vessels (posterior cardinal vein, PCV) in blue. (A) Measurements of cardiac parameters in desma^sa5-/-, ct122aGt, ct122aRGt and control embryos including heart rate and fractional shortening analysis. The fractional shortening (FS%) is calculated as the ratio of the difference between diastolic and systolic chamber diameter over the diastolic diameter. Scale bar=30μm. (B) Zoom on the DA and PCV showing the flowing red blood cells, which were tracked to measure the blood flow velocity. The graph shows the relative maximal blood flow velocity measured in the DA of the trunk as readout of the pumping efficiency in desmasa5-/- and in ct122aGt embryos compared to their relative controls. Scale bar=20μm. (C) Z-stack maximal projections showing the organization of the intersegmental vessels (ISVs) in desma^sa5+/+, desma^sa5-/-; ct122aGt positive and control Tg(fli:gal4FF^ubs; UAS:kaede) embryos at 50hpf. Scale bar =20μm. (D) Percentage of circulating versus non-circulating vessels desma^sa5-/-, ct122aGt and ct122aRGt embryos compared to control embryos. (E) Measurements of ISV diameters show a higher variability in desma^sa5-/- and ct122aGt compared to their corresponding controls. ct122aGt and ct122aRGt indicate desma^ct122aGt and desma^ct122aRGt homozygous embryos. Error bars indicate the standard deviation.