ZFIN ID: ZDB-FIG-170220-34
Wild et al., 2017 - Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization. Nature communications   8:13991 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 4

Arterial and venous-derived endothelial cells populate the dorsal part of venous ISV and contribute to sprouting upon loss of flt1.

(a) Endothelial cell tracking in Tg(kdrl:nlsKikGR)hsc7 embryos. Photo-converted PCV-derived venous endothelial cells express red-kikGR. (bf) Endothelial cell tracing during 30–60 hpf, showed that PCV-derived endothelium, indicated in red & labelled a,b,c,d,e, migrated along the ISV, from ventral to dorsal up to the most dorsal part of vISVs (cell labelled b). Pre-existing arterial endothelial cells in ISV, in green and labelled 1,2,3,4, shows artery-derived ECs in the dorsal part of vISVs (cell labelled 2) adjacent of PCV-derived EC (cell labelled b,a). (g) Schematic representation of the scenario imaged in bf. (h) Left panel: Identity analysis of endothelial cells in the dorsal part of vISV revealed three different scenarios: (I) mixed, both arterial and venous-derived endothelium were present, (II) only vein-derived endothelium, (III) only artery-derived endothelium. (n=10 experiments & 6ISVs/embryo). Right panel: % of artery and vein-derived endothelium in the mixed population scenario. (i) Schematic representation of the three identity scenarios in dorsal part of vISV. Arterial derived EC in red, venous-derived EC in blue. (jl) Ectopic sprouting scenarios in flt1 morphants in Tg(flt1enh:Tdtomato; flt4:mCitrine), n=6 embryos. (j) Ectopic venous sprout devoid of flt1enh expressing artery-derived EC (arrowhead). (k) Ectopic venous sprout (arrowhead) containing both arterial and venous-derived ECs; the flt1enh expressing artery-derived EC is juxtaposed to the venous-derived cell at the tip (arrowhead). (l) Ectopic venous sprout only containing flt1enh expressing artery-derived ECs; flt1enh (red) and flt4 (green) were expressed by the same cell which appears in yellow (arrowhead). (mo) Schematic representation of the three ectopic venous sprouting scenarios. (p) Ectopic sprouting upon loss of flt1 in Tg(flt1enh:Tdtomato; flt4:mCitrine) (representative of 5 embryos). Flt1enh positive sprouts (pink arrowhead) and sprouts devoid of flt1enh (white arrowhead). MO, flt1 morpholino, 1ng. aISV, arterial intersegmental vessel; DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; PCV, posterior cardinal vein; vISV, venous intersegmental vessel. Scale bar, 20 μm in jl, 10 μm in bf,p.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine hu5333Tg; hu7135Tg + MO3-flt1 standard conditions Protruding-mouth dorsal longitudinal anastomotic vessel IFL
Protruding-mouth intersegmental vessel IFL
Day 4 intersegmental vessel IFL
Day 4 posterior cardinal vein IFL
Day 4 trunk vasculature IFL
KikGR hsc7Tg standard conditions Prim-15 to Pec-fin dorsal aorta IFL
Prim-15 to Pec-fin dorsal longitudinal anastomotic vessel IFL
Prim-15 to Pec-fin intersegmental artery IFL
Prim-15 to Pec-fin intersegmental vein IFL
Prim-15 to Pec-fin intersegmental vessel IFL
Prim-15 to Pec-fin posterior cardinal vein IFL
RFP hu5333Tg; hu7135Tg + MO3-flt1 standard conditions Protruding-mouth dorsal longitudinal anastomotic vessel IFL
Protruding-mouth intersegmental vessel IFL
Day 4 dorsal aorta IFL
Day 4 intersegmental vessel IFL
Day 4 trunk vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hu5333Tg; hu7135Tg + MO3-flt1 standard conditions Protruding-mouth intersegmental vessel vascular sprouts increased amount, abnormal
Day 4 intersegmental vessel vascular sprouts increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.