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ZIRC
ZFIN ID: ZDB-FIG-170209-14
Sotolongo-Lopez et al., 2016 - Genetic Dissection of Dual Roles for the Transcription Factor six7 in Photoreceptor Development and Patterning in Zebrafish. PLoS Genetics   12:e1005968 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. 1

ljrp23ahub mutants display an increased number and uniform distribution of rod photoreceptors.

(A) Confocal immunofluorescent images labeled for rods (red) and UV-sensitive cones (green) from WT, ljrp23ahub, lorp25bbtl and homozygous lorp25bbtl/ljrp23ahub retinas at 4 days-post-fertilization (dpf). WT larvae show asymmetric rod distribution in central and dorsal retina and a uniform distribution of UV-sensitive cones across the entire retina. ljrp23ahub and lorp25bbtl mutants demonstrate an increased number and uniform arrangement of rods but fewer UV-sensitive cones labeled in lorp25bbtl. Double-mutant retinas display higher rod labeling and few UV-sensitive cones. (B) Graph showing the average number of rods per unit area dorsal to the optic nerve (WT, n = 5; ljrp23ahub, n = 5; lorp25bbtl, n = 5; ljrp23ahub/lorp25bbtl, n = 5). Significantly-different means by one-way ANOVA with Tukey’s post-hoc test, b vs c, p<0.05, all other comparisons p<0.0001. (C) Graph showing the average number of UV-sensitive cones per unit area from samples used in 1B, a vs b, p<0.0001. (D) Graph showing the average number of rods per unit area (WT, n = 5; ljrp23ahub/+, n = 6; ljrp23ahub, n = 5); a vs b, p<0.05, all other comparisons p<0.0001. (E) Flat mount views of confocal immunofluorescent images labeled for red-sensitive (brighter signal) and green -sensitive (dimmer signal) cones from WT and ljrp23ahub retinas at 4 dpf. ljrp23ahub mutants maintain the alternating arrangement of red- and green-sensitive cones. (F) Graph showing the average number of red- and green-sensitive cones per unit area (WT, n = 6; ljrp23ahub, n = 6). No significant differences are observed, Student’s t test, p>0.05. Error bars represent SD.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-4C12 IHC WT standard conditions Day 4 retinal rod cell
IHC ljrp23ahub/p23ahub standard conditions Day 4 retinal rod cell
IHC tbx2bp25bbtl/+ ; ljrp23ahub/+ standard conditions Day 4 retinal rod cell
IHC tbx2bp25bbtl/p25bbtl standard conditions Day 4 retinal rod cell
Ab1-opn1sw1 IHC WT standard conditions Day 4 UV sensitive photoreceptor cell
IHC ljrp23ahub/p23ahub standard conditions Day 4 UV sensitive photoreceptor cell
IHC tbx2bp25bbtl/+ ; ljrp23ahub/+ standard conditions Day 4 UV sensitive photoreceptor cell
IHC tbx2bp25bbtl/p25bbtl standard conditions Day 4 UV sensitive photoreceptor cell
Phenotype Details
Fish Conditions Stage Phenotype
ljrp23ahub/+ standard conditions Day 4 retinal rod cell increased amount, abnormal
ljrp23ahub/p23ahub standard conditions Day 4 retinal rod cell increased amount, abnormal
Day 4 retinal rod cell spatial pattern, abnormal
tbx2bp25bbtl/+ ; ljrp23ahub/+ standard conditions Day 4 retinal rod cell increased amount, abnormal
Day 4 retinal rod cell spatial pattern, abnormal
Day 4 UV sensitive photoreceptor cell decreased amount, abnormal
tbx2bp25bbtl/p25bbtl standard conditions Day 4 retinal rod cell increased amount, abnormal
Day 4 UV sensitive photoreceptor cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.