FIGURE

Fig. 3

ID

ZDB-FIG-170103-5

Publication

Perens et al., 2016 - Hand2 inhibits kidney specification while promoting vein formation within the posterior mesoderm

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Fig. 3

Pronephron development is susceptible to hand2 overexpression prior to the 10 somite stage.

(A–E) Dorsal views, anterior to the left, of in situ hybridization for atp1a1a.4 at 24 hpf depict a range of severity of pronephron defects, ranging from absence of the pronephron (A) to unaffected (E). Tg(hsp70:FLAG-hand2-2A-mCherry) embryos were subjected to heat shock at the tailbud, 2 somite, 6 somite, or 10 somite stages, and the consequences on pronephron development were scored at 24 hpf. Percentages indicate the distribution of phenotypes produced by each treatment; the number of embryos examined is in the right-hand column. Heat shock at later stages resulted in more mild loss of atp1a1a.4 expression in the tubule, and heat shock at the 10 somite stage did not disrupt atp1a1a.4 expression in the tubule. Scale bar represents 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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