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ZFIN ID: ZDB-FIG-161206-5
Zhang et al., 2016 - Inhibition of vascular endothelial growth factor signaling facilitates liver repair from acute ethanol-induced injury in zebrafish. Disease models & mechanisms   9(11):1383-1396 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 6

Fig. 1

VEGFR inhibition suppressed the increase of HSC cell number post-acute ethanol exposure. (A) Work flow of the ethanol and VEGF receptor (VEGFR) inhibitor treatment. (B-E) Representative confocal single-plane images of Tg(hand2:EGFP;kdrl:ras-mCherry) larvae treated with 0.03% DMSO (B), 2% ethanol followed by DMSO (C), 3 μM VEGFR inhibitor ZM (D), and 2% ethanol followed by 3 μM ZM (E) at 27 h post-ethanol treatment (hpt). The HSCs are labeled by Tg(hand2:EGFP) expression (green), and the endothelial cells are marked by Tg(kdrl:ras-mCherry) expression (red). Arrows point to the cellular processes of HSCs. Ventral view, anterior is to the top. Sample sizes are indicated. A, anterior; R, right. Scale bar: 50 μm. (F-I) Confocal three-dimensional projections of vibratome transverse sections stained by TUNEL assay. The apoptotic cells are shown in red (arrowheads), and the HSCs are labeled by Tg(hand2:EGFP) expression (green). Very few apoptotic cells were present in the livers of all the control and experimental groups, but were frequently seen in the intestine (int). For each group, three liver sections per larva from 10 larvae were analyzed. Dashed line marks the liver. Scale bar: 30 μm. (J) Numbers (mean±s.e.m.) of HSCs per liver in each treatment group at 27 hpt. (K) Changes in the numbers (mean±s.e.m.) of HSCs per liver in each treatment group between 0 and 27 hpt. The numbers of animals analyzed are indicated in J and Fig. S1F. (L) Percentages (mean±s.e.m.) of HSCs that incorporated EdU labeling in each treatment group. (J-L) Each experiment was repeated at least three times and the numbers of animals analyzed are shown. Statistical significance in J was calculated by one-way ANOVA and Tukey's post-hoc test; in L it was determined by two-tailed Student's t-test. ***P<0.001; ****P<0.0001; ns, not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s843Tg ; s896Tg control Day 6 ito cell IFL
s843Tg ; s896Tg chemical treatment by environment: ethanol, chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 ito cell IFL
s843Tg ; s896Tg chemical treatment by environment: ethanol Day 6 ito cell IFL
s843Tg ; s896Tg chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 ito cell IFL
mCherry s843Tg ; s896Tg control Day 6 liver endothelial cell IFL
s843Tg ; s896Tg chemical treatment by environment: ethanol, chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 liver endothelial cell IFL
s843Tg ; s896Tg chemical treatment by environment: ethanol Day 6 liver endothelial cell IFL
s843Tg ; s896Tg chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 liver endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s843Tg ; s896Tg chemical treatment by environment: ethanol, chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 ito cell cell projection decreased length, ameliorated
Day 6 liver ito cell apoptotic, normal
s843Tg ; s896Tg chemical treatment by environment: ethanol, chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 ito cell cell projection decreased length, abnormal
s843Tg ; s896Tg chemical treatment by environment: ethanol, chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 liver has normal numbers of parts of type ito cell, normal
s843Tg ; s896Tg chemical treatment by environment: ethanol, chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 liver ito cell apoptotic, normal
s843Tg ; s896Tg chemical treatment by environment: ethanol, chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 liver ito cell proliferative, ameliorated
s843Tg ; s896Tg chemical treatment by environment: ethanol Day 6 ito cell cell projection decreased length, abnormal
Day 6 liver has extra parts of type ito cell, abnormal
Day 6 liver has normal numbers of parts of type ito cell, normal
Day 6 liver ito cell apoptotic, normal
Day 6 liver ito cell proliferative, abnormal
s843Tg ; s896Tg chemical treatment by environment: N-(4-chloro-2-fluorophenyl)-6,7-dimethoxy-4-quinazolinamine Day 6 ito cell morphology, normal
Day 6 liver has normal numbers of parts of type ito cell, normal
Day 6 liver ito cell apoptotic, normal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.