ZFIN ID: ZDB-FIG-161101-3
Poon et al., 2016 - Development of the cardiac conduction system in zebrafish. Gene expression patterns : GEP   21(2):89-96 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Days 30-44 to Adult

Fig. 3

The EGFP expression at the ring of cells at the SAN and AVC is maintained into adulthood in the CCS transgenics. A: A cryosection of a one mpf CCS EGFP + cells stained with anti-acetylated tubulin (red), anti-EGFP (green) and counterstaining with DAPI (blue) shows that the SAR pacemaker cells are neuronally innervated. A′: Enlargement of inset from A - fluorescence at the atrium base. B: In the adult heart, the EGFP expression is localized to the ring of cells located at the SAR and AVR as well as cellular connection between SAN and AVC (labeled by orange arrow). C-C′: Adult whole heart double stained with anti-EGFP (green) and anti-acetylated tubulin (red) shows nerve fibers extending from SAN towards AVC, in close proximity to EGFP cells (green arrows). D: Confocal image of SAN ring showing multiple contacts (blue asterisks) with SAN conduction cells ring. All arrows in this Fig points to SAN EGFP cells whereas arrowheads point to AVC EGFP cells. Dotted white lines in A outline the chambers of the adult zebrafish heart. Abbreviations: A, atrium; AVC, atrio-ventricular region; OFT, outflow tract; SAN, sino-atrial region; SV, sinus venosus; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP sqet33mi28Et standard conditions Days 30-44 atrioventricular node IFL
Days 30-44 sinoatrial node IFL
Adult atrioventricular node IFL
Adult sinoatrial node IFL
sqet33mi59BEt standard conditions Days 30-44 atrioventricular node IFL
Days 30-44 sinoatrial node IFL
Adult atrioventricular node IFL
Adult sinoatrial node IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Gene expression patterns : GEP, 21(2), Poon, K.L., Liebling, M., Kondrychyn, I., Brand, T., Korzh, V., Development of the cardiac conduction system in zebrafish, 89-96, Copyright (2016) with permission from Elsevier. Full text @ Gene Expr. Patterns