FIGURE

Fig. 1

ID
ZDB-FIG-160921-16
Publication
Qu et al., 2016 - Axitinib blocks Wnt/β-catenin signaling and directs asymmetric cell division in cancer
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Fig. 1

Axitinib inhibits Wnt/β-catenin signaling in zebrafish. (A) Chemical structure of axitinib. (B) Zebrafish embryos at 6 h post fertilization (hpf) were treated with 6BIO and axitinib at the indicated concentrations, and the eyeless phenotype was assessed 24 h later. n = total number of assessed embryos over four independent experiments. Embryos that died during treatment (n = 0, 4, 2, 0, 1, and 2 in the groups from left to right) were excluded from assessment. (C, Left) Representative images at 28 hpf of TCF-GFP transgenic zebrafish embryos treated with DMSO or axitinib (5 µM) for 2 d. n = 40 in each group; no embryos died. (Right) Representative images at 76 days post fertilization (dpf). The middle-hindbrain boundary (white arrows) and caudal fin mesenchyme are enlarged at the left and right, respectively. (D) Representative images of tailfin regeneration in TCF-GFP transgenic zebrafish (13 wk, n = 5 fish per group) treated with DMSO or axitinib (5 µM) for 6 d post amputation (dpa). Scale bars, 200 µm.

Expression Data
Gene:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-5 to Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Observed In:
Stage Range: Prim-5 to Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA