ZFIN ID: ZDB-FIG-160831-11
Xu et al., 2016 - Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine. Developmental Cell   38(2):214-22 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Pec-fin to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 3

Neuron-Specific bcl-2 Overexpression Suppresses Neuronal Cell Death and Blocks Microglia Colonization of the Optic Tectum

(A) Acridine orange (AO) staining reveals that neuronal cell death is largely prevented in the optic tectum of 3 dpf Tg(Xla.Tubb:bcl-2) embryos. The optic tectum is indicated by dashed lines.

(B) The number of microglia in the optic tectum is drastically reduced in the optic tectum of 3 dpf Tg(Xla.Tubb:bcl-2) embryos. The optic tectum is indicated by dashed lines. Red and green signals represent coro1a-DsRedx+ microglia and elavl3-GFP+ neurons, respectively.

(C) Quantification of the number of microglia in the forebrain, midbrain, and hindbrain of 3 dpf WT and Tg(Xla.Tubb:bcl-2) embryos. Error bars represent the mean ± SEM. ***p < 0.001 (n = 4).

(D) Quantification of the number of microglia in the optic tectum and ventral midbrain of 3 dpf WT and Tg(Xla.Tubb:bcl-2) embryos. Error bars represent the mean ± SEM. **p < 0.01 (n = 4).

(E) Quantification of the number of microglia that migrate into the optic tectum within the 24 hr imaging period (from 2 dpf to 3 dpf) of WT and Tg(Xla.Tubb:bcl-2) embryos. Error bars represent the mean ± SEM. ***p < 0.001 (n = 5 for WT embryos, n = 4 for transgenic embryos).

(F) Quantification of the percentage of microglia that shuffle out of the optic tectum within the 24 hr imaging period (from 2 dpf to 3 dpf) of WT and Tg(Xla.Tubb:bcl-2) embryos. Error bars represent the mean ± SEM. **p < 0.01 (n = 5 for WT embryos, n = 4 for transgenic embryos).

(G) Coronal (top) and transverse (bottom) views of time-lapse imaging pictures of the midbrain show a typical microglia (labeled by the white arrow) that enters the optic tectum and subsequently migrates out of the optic tectum in the Tg(Xla.Tubb:bcl-2) embryos. The optic tectum and eyes are indicated by dashed lines. Red and green signals represent coro1a-DsRedx+ microglia and elavl3-GFP+ neurons, respectively. E, eye; OT, optic tectum.

See also Figure S3 and Movie S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRedx hkz011tTg; hkz013tTg; tsu11Tg standard conditions Pec-fin microglial cell IFL
Pec-fin optic tectum microglial cell IFL
Protruding-mouth microglial cell IFL
Protruding-mouth optic tectum microglial cell IFL
hkz011tTg; tsu11Tg standard conditions Protruding-mouth microglial cell IFL
Protruding-mouth optic tectum microglial cell IFL
EGFP hkz011tTg; hkz013tTg; tsu11Tg standard conditions Pec-fin brain neuron IFL
Pec-fin optic tectum neuron IFL
Protruding-mouth optic tectum neuron IFL
hkz011tTg; tsu11Tg standard conditions Protruding-mouth optic tectum neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hkz011tTg; hkz013tTg; tsu11Tg standard conditions Protruding-mouth midbrain microglial cell decreased amount, abnormal
Protruding-mouth optic tectum microglial cell decreased amount, abnormal
Protruding-mouth optic tectum microglial cell migration process quality, abnormal
hkz013tTg standard conditions Protruding-mouth optic tectum neuron death decreased occurrence, abnormal
Acknowledgments:
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Reprinted from Developmental Cell, 38(2), Xu, J., Wang, T., Wu, Y., Jin, W., Wen, Z., Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine, 214-22, Copyright (2016) with permission from Elsevier. Full text @ Dev. Cell