ZFIN ID: ZDB-FIG-160726-9
Hensley et al., 2016 - Evolutionary and developmental analysis reveals KANK genes were co-opted for vertebrate vascular development. Scientific Reports   6:27816 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Long-pec

Fig. 4

Kank2, kank3 and kank4 gene expression during zebrafish development.

Whole mount in situ hybridization of zebrafish embryos at stages 15 hpf (a,b,j,k,s,t), 18 hpf (v), 24 hpf (c-e,l,m,u) and 48 hpf (f-i,n-q,w-z′). Anterior is to the left in all whole-mount images, and dorsal is to the top in all transverse sections. (a-i). Gene expression of kank2. (j-r). Gene expression of kank3. (s-z′). Gene expression of kank4. The dashed lines indicate the positions of section. The letters below the dashed lines correspond to the panels. The inset within panel (u) is the transverse section of the dashed line above. The white arrow points to the kank4 expression on the vascular vessels on the surface of the yolk. af, anal fin bud; cf, caudal fin bud; cv, caudal vein; da, dorsal aorta; df, dorsal fin bud; dlv, dorsal longitudinal vein; e, eye; hb, hindbrain; hv, head vessels; llp, lateral line premordia; psm, presomatic mesoderm; lpm, lateral plate mesoderm; mb, midbrain; nt, neural tube; ov, otic vesicle; pa, pharyngeal arch; pcv, posterior cardinal vein; pf, pectoral fin bud; so, somite.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
kank2 WT standard conditions 10-13 somites head blood vessel ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
Prim-5 head blood vessel ISH
Prim-5 lateral line primordium ISH
Prim-5 midbrain ISH
Prim-5 trunk vasculature ISH
Long-pec head blood vessel ISH
Long-pec hindbrain ISH
Long-pec midbrain ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
kank3 WT standard conditions 10-13 somites head blood vessel ISH
Prim-5 caudal vein plexus ISH
Prim-5 dorsal aorta ISH
Prim-5 fin bud ISH
Prim-5 head blood vessel ISH
Long-pec caudal fin ISH
Long-pec caudal vein plexus ISH
Long-pec dorsal aorta ISH
Long-pec head blood vessel ISH
kank4 WT standard conditions 10-13 somites lateral plate mesoderm ISH
10-13 somites otic placode ISH
14-19 somites hindbrain ISH
14-19 somites vascular cord ISH
Prim-5 head blood vessel ISH
Prim-5 otic vesicle ISH
Prim-5 posterior cardinal vein ISH
Long-pec anal fin ISH
Long-pec caudal fin ISH
Long-pec head blood vessel ISH
Long-pec midbrain ISH
Long-pec otic vesicle ISH
Long-pec pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.