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ZIRC
ZFIN ID: ZDB-FIG-160525-27
Zhao et al., 2016 - An essential role for Grk2 in Hedgehog signalling downstream of Smoothened. EMBO reports   17(5):739-52 Full text @ EMBO Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 3

Grk2 acts upstream of Gli processing

A. Prox1a and Eng expression in the myotome of 30hpf wildtype (WT) and MZgrk2 embryos injected with mRNAs encoding Shh, dnPKA or mSmoA1GFP. Each panel shows Prox1a in green, Eng in red and the merged images with DAPI staining in blue. The colabelling for Prox1a and Eng (orange) is indicative of MP differentiation (n = 30 for each sample). Scale bar, 50 ┬Ám.

B. Western blot showing levels of Gli2aFL and Gli2aR forms in 20hpf wildtype (WT) and MZgrk2 (MZ) embryos injected with mRNA encoding Shh, dnPKA or mSmoA1GFP, respectively. γ-tubulin was used as a loading control. Three biological replicates of this analysis were performed.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-eng IHC WT standard conditions Prim-15 myotome fast muscle cell
IHC Prim-15 myotome muscle pioneer
IHC grk3i283/i283 standard conditions Prim-15 Not Detected myotome fast muscle cell
IHC Prim-15 Not Detected myotome muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
grk3i283/i283 standard conditions Prim-15 myotome fast muscle cell ab1-eng labeling absent, abnormal
Prim-15 myotome fast muscle cell absent, abnormal
Prim-15 myotome muscle pioneer ab1-eng labeling absent, abnormal
Prim-15 myotome muscle pioneer absent, abnormal
Prim-15 slow muscle cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal EMBO reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ EMBO Rep.