ZFIN ID: ZDB-FIG-160518-14
Eden et al., 2016 - Myoscape controls cardiac calcium cycling and contractility via regulation of L-type calcium channel surface expression. Nature communications   7:11317 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. S6

(a left panel) RT -PCR analysis in zebrafish hearts from Myoscape knockdown morphants compared to controls showed a successful splice site mutation as indicated by a clear double band. (a right panel) Western blot analysis of zebrafish hearts from Myoscape knockdown morphants compared to controls, revealing a significant (>60%) reduction of Myoscape protein content after morpholino injection. (b+d) Histological (HE staining, scale bar 100µm), immunohistochemical (scale bar 100µm) (c) and ultrastructural evaluation of zebrafish heart (e) sections and skeletal muscle tissue (f) showing no gross architectural deficits after Myoscape ablation, indicating no obvious role of Myoscape in development or cellular sub-architecture of these tissues in zebrafish (scale bar EM 1 µm).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
strip2 WT standard conditions Long-pec whole organism RTPCR
Long-pec whole organism WB
WT + MO1-strip2 standard conditions Long-pec whole organism RTPCR
Long-pec whole organism WB
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
IHC WT + MO1-strip2 standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
Ab-S46 IHC WT standard conditions Long-pec atrial myocardium
IHC WT + MO1-strip2 standard conditions Long-pec atrial myocardium
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-strip2 standard conditions Long-pec whole organism strip2 expression decreased amount, abnormal
Long-pec whole organism strip2 expression decreased amount, abnormal
Protruding-mouth cardiac atrium morphogenesis process quality, normal
Protruding-mouth cardiac muscle cell differentiation process quality, normal
Protruding-mouth cardiac ventricle morphogenesis process quality, normal
Protruding-mouth myocardium sarcoplasmic reticulum morphology, normal
Protruding-mouth myocardium T-tubule morphology, normal
Protruding-mouth myocardium Z disc morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.