Fig. 5 S3

The ARTR is recruited by whole-field motion.

(A) Laterality tuning map for one fish, showing the characteristic long-timescale correlations with fictive behavior (B). This map is used to choose left and right ARTR ROIs that are used to assay ARTR responses to different directions of whole-field motion (C). (D) Responses of the ARTR ROIs to the eight directions of motion indicated in (C). This periodic motion paradigm drives turning strongly for orthogonal directions of motion and drives ARTR activity periodically, with the left ARTR activated for stimuli oriented to the left and the right ARTR activated for stimuli oriented to the right. (E) Experimental paradigm designed to test the effect of ARTR activation by whole-field motion. Freely swimming larvae are presented with drifting gratings moving orthogonal to the body axis, either to the left or the right, depending on the trial. These gratings are presented with closed-loop feedback such that they are always locked to the fish’s body axis even as the fish swims around the petri dish. This stimulus drives turning in the direction of motion. After 10.5 s of optic flow, the gratings fade away linearly over 2 s, and behavior is monitored under subsequent spontaneous conditions for 8.5 s. (F) The probability of turning in the direction of flow reaches a maximum during the flow and fade epochs but stays elevated above chance for three bouts into the null epoch. (G) The direction of the first swim bout following visual stimulation is strongly related to the amount of turning during optic flow. The more the fish turns during optical stimulation, the higher the chance that the first swim bout in the blank period is in the same direction (22 fish) Figure 6G-H.