Fig. 2

Retinal broad-spectrum MMP inhibition after ONC reduces OT reinnervation. A-D: Representative images depicting reinnervation of the OT by RGC axons at 7 dpi after PBS, DMSO, or GM6001 treatment, reveal a clearly diminished reinnervated OT area after broad-spectrum matrix metalloproteinase (MMP) inhibition (see arrows) as opposed to vehicle control and UCC fish. E: Quantification of the area covered by RGC axons in the OT of the various treatment conditions, relative to UCC fish. Both DMSO- and PBS-treated fish show 60% reinnervation of the OT relative to the completely innervated OT in the UCC condition. A significant reduction in tectal reinnervation was noted in GM6001-treated fish compared to the vehicle controls. Data represent mean ± SEM and 8-15 animals were used per condition over three independent experiments (*P < 0.05; **P < 0.005; ***P < 0.001). UCC, uncrushed control; PBS, phosphate-buffered saline; DMSO, dimethyl sulfoxide. Scale bars = 200 µm.