ZFIN Figure: Burns et al., 2016, Fig. 2

ZFIN ID: ZDB-FIG-160304-52

ZFIN ID: ZDB-FIG-160304-52

Burns et al., 2016 - Analysis of the Zebrafish sox9b Promoter: Identification of Elements that Recapitulate Organ-Specific Expression of sox9b. Gene 578(2):281-9 Full text @ Gene

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	uw102Tg (AB) uw103Tg (AB) uw104Tg (AB) uw105Tg (AB) uw106Tg (AB)
Anatomical Terms:	brain heart mandibular arch skeleton muscle cell notochord skeletal muscle
Stage:	Day 4

Fig. 2

Expression constructs and summary of expression. (A) Expression constructs with different lengths of the sox9b promoter. The founders obtained for each segment are designated as lines A,B,C,D, or E. (B) Lateral images of 96 hpf zebrafish from each stable transgenic line, using confocal microscopy showing EGFP reporter expression as green fluorescence. Abbreviations: brain, b; heart, h; jaw, j; skeletal muscle, m; and notochord, n. The + indicates consistently observed; the indicates consistently not observed; +/ indicates sporadic or weak occurrence in offspring.

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
EGFP	uw102Tg (AB)	standard conditions	Day 4		brain	IFL
			Day 4		heart	IFL
			Day 4		mandibular arch skeleton	IFL
			Day 4		muscle cell	IFL
			Day 4		notochord	IFL
			Day 4		skeletal muscle	IFL
	uw103Tg (AB)	standard conditions	Day 4		brain	IFL
			Day 4		heart	IFL
			Day 4		mandibular arch skeleton	IFL
			Day 4		notochord	IFL
			Day 4		skeletal muscle	IFL
	uw104Tg (AB)	standard conditions	Day 4		brain	IFL
			Day 4		heart	IFL
			Day 4		mandibular arch skeleton	IFL
			Day 4		notochord	IFL
			Day 4		skeletal muscle	IFL
	uw105Tg (AB)	standard conditions	Day 4		brain	IFL
			Day 4		heart	IFL
			Day 4	Not Detected	mandibular arch skeleton	IFL
			Day 4	Not Detected	notochord	IFL
			Day 4		skeletal muscle	IFL
	uw106Tg (AB)	standard conditions	Day 4	Not Detected	brain	IFL
			Day 4		heart	IFL
			Day 4	Not Detected	mandibular arch skeleton	IFL
			Day 4	Not Detected	notochord	IFL
			Day 4	Not Detected	skeletal muscle	IFL

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Gene, 578(2), Burns, F.R., Lanham, K.A., Xiong, K.M., Gooding, A.J., Peterson, R.E., Heideman, W., Analysis of the Zebrafish sox9b Promoter: Identification of Elements that Recapitulate Organ-Specific Expression of sox9b, 281-9, Copyright (2016) with permission from Elsevier. Full text @ Gene

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Gene, 578(2), Burns, F.R., Lanham, K.A., Xiong, K.M., Gooding, A.J., Peterson, R.E., Heideman, W., Analysis of the Zebrafish sox9b Promoter: Identification of Elements that Recapitulate Organ-Specific Expression of sox9b, 281-9, Copyright (2016) with permission from Elsevier. Full text @ Gene