FIGURE

Fig. S2

ID

ZDB-FIG-160212-53

Publication

Beerman et al., 2015 - Direct In Vivo Manipulation and Imaging of Calcium Transients in Neutrophils Identify a Critical Role for Leading-Edge Calcium Flux

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Fig. S2

(Relates to Figure 1). Detailed analysis of calcium activity profile from a migrating neutrophil.

The top image of a cell illustrates how the boxes L=4, 20, 36, 52, 68, 84, and 100 from Figure S1 were rotated, stretched, and magnified to highlight the enriched calcium flux at the leading edge, and to a lesser extent the lagging edge over time. The y axis is time (min) and the x axis is the GCaMP3/dsRed ratio oriented such that the turquoise line shows a visual threshold of the ratio at 15 and the dark-blue line shows a visual threshold of the ratio at 10. The time-lapse ratiometric cell images on the left correspond approximately to the time axis on the calcium activity profiles to provide a visual readout of the quantified signal graphed adjacently.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.