FIGURE

Fig. S2

ID

ZDB-FIG-151008-4

Publication

Aspatwar et al., 2015 - Inactivation of ca10a and ca10b Genes Leads to Abnormal Embryonic Development and Alters Movement Pattern in Zebrafish

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Fig. S2

Silencing of egfp expression in tg(fli1a:egfp) zebrafish embryos using CRISPR/Cas9 mediated mutagenesis.

A) Fluorescence microscopy was used to analyze the silencing of egfp in tg(fli1a:egfp) zebrafish embryos at 2 dpf. The un-injected control (left) and egfp gRNA injected control (middle) express egfp (green) in the vascular endothelium. The CRISPR-Cas9 mutated embryo (right) shows less fluorescence due to the disruption of the egfp gene. The red channel was used to detect auto-fluorescence. B) T7 endonuclease I (T7EI) assay was used to evaluate the egfp mutation efficiency in 2-day-old embryos. T7EI treated PCR products of un-injected and egfp gRNA injected control fish are shown in comparison to PCR products of two individual embryos and a pooled sample of 5 egfp silenced embryos. The full length wild type (WT) egfp product (470bp) is marked with an asterix. Arrows indicate the T7E1 cleaved PCR products in the egfp mutated embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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