ZFIN ID: ZDB-FIG-150915-42
Caspase 3/7 activation is increasingly impaired in RP mutants.
A) Caspase 2 and B) 3/7 activity is measured using a proluminescent caspase-3/7 DEVD-aminoluciferin substrate in 2 dpf embryos either untreated or exposed to 25 Gy ionizing radiation (IR). C) TUNEL analysis of 2 dpf wild type or rpS7 embryos reveals cells with DNA fragmentation when embryos are exposed to 25 Gy IR. D) The number of TUNEL-positive cells in (C) are quantified. E) TUNEL-positive cells in both wild type and rpS7 mutants are observed predominantly in the brain region (arrowhead) or in the tail tissue dorsal to the notochord (black box). *p<0.05, **p<0.01.
Gene Expression DetailsNo data available
Antibody Labeling Details No data available
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