FIGURE

Fig. 4

ID

ZDB-FIG-150820-4

Publication

LeBert et al., 2015 - Matrix metalloproteinase 9 modulates collagen matrices and wound repair

Other Figures

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Fig. 4

Acute wounding induces NFκB-dependent expression of mmp9. (A) An increased expression of mmp9 is observed by 6h post wounding (hpw) in the 2-dpf embryo. (B) A visible increase in NFκB activation is seen by 6hpw using the transgenic Tg(NFκB:gfp). The star denotes neuromast cells expressing high levels of gfp independent of wounding. The arrow indicates the edge of the wounded caudal fin and the site of increased NFκB activation. (C) Ratiometric image analysis performed in a cross of Tg(NFκB:gfp) and Tg(krt4:tdTom). (D) Early inhibition of the NFκB pathway with 30µM withaferin A resulted in a significant reduction in regeneration at 3dpw but had no effect on developmental fin length. (E) Early inhibition of the NFκB pathway resulted in an abrogation of the increased mmp9 expression in the Tg(krt4:l10a-gfp) TRAP line. *P<0.05, **P<0.01 and ****P<0.0001. Scale bars: 40µm in B,C; 100 µm in D. A represents data combined from experiments performed in triplicate; D represents data combined from experiments performed in quadruplicate and normalized to unwounded treatments.

Expression Data

Gene:	EGFP
Fish:	nc1Tg nc1Tg; uwm17Tg
Condition:	physical alteration: caudal fin
Anatomical Terms:	fin neuromast regenerating fin
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT nc1Tg nc1Tg; uwm17Tg
Conditions:	physical alteration: caudal fin chemical treatment: withaferin A, physical alteration: caudal fin
Observed In:	activation of NF-kappaB-inducing kinase activity regenerating fin
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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