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ZFIN ID: ZDB-FIG-150811-33
Vanhollebeke et al., 2015 - Tip cell-specific requirement for an atypical Gpr124- and Reck-dependent Wnt/β-catenin pathway during brain angiogenesis. eLIFE   4 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to High-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to High-pec

Fig. 6 Tip cell defects in Wnt/β-catenin-deficient PHBCs.

(A) Scatterplot of a microarray comparison of wild-type and gpr124s984/s984 embryos at 48 hpf. Each dot refers to the relative signal intensity of a given probe in mutant vs wild-type embryos. Circles identify independent probes for vegfaa, vegfab, and vegfc. (B) Endothelial cell number in the PHBCs of wild-type and gpr124s984/s984 embryos at 30 hpf. kdrl:NLS-GFP+ nuclei were counted on one side of the embryos. (C) Dorsal views of Tg(pdgfrb:mCitrine);Tg(kdrl:ras-mCherry) wild-type and gpr124s984/s984 embryos at 40 hpf. (D) Stills from Video 3 recording sprouting angiogenesis in the hindbrain of Tg(kdrl:GFP) wild-type and gpr124s984/s984 embryos. Below are kymograph plots showing a time course of pixel intensity across the yellow virtual lines running 15 µm dorsal to the PHBC (from the anterior MCeV to the posterior PCeV [posterior cerebral vein]) and depicted in the upper left panel. (E) Lateral views of Tg(kdrl:lifeact-GFP);Tg(kdrl:NLS-mCherry) wild-type and gpr124s984/s984 hindbrains at 30 hpf. Arrows point to actin-dense structures forming in the PHBC vessel wall. Scale bars, 50 µm. Values represent means ± SD.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
Citrine adgra2s984/s984; s1010Tg; s896Tg standard conditions Prim-25 pericyte IFL
Prim-25 posterior communicating artery anatomical region IFL
Prim-25 posterior communicating artery pericyte IFL
s1010Tg; s896Tg standard conditions Prim-25 pericyte IFL
Prim-25 posterior communicating artery anatomical region IFL
Prim-25 posterior communicating artery pericyte IFL
EGFP adgra2s984/s984; is4Tg; s982Tg standard conditions Prim-15 endothelial cell filamentous actin IFL
Prim-15 primordial hindbrain channel filamentous actin IFL
adgra2s984/s984; s843Tg standard conditions Prim-15 mid cerebral vein IFL
Prim-15 primordial hindbrain channel IFL
Prim-15 Not Detected primordial hindbrain channel angiogenic sprout IFL
Prim-25 mid cerebral vein IFL
Prim-25 primordial hindbrain channel IFL
Prim-25 Not Detected primordial hindbrain channel angiogenic sprout IFL
High-pec mid cerebral vein IFL
High-pec primordial hindbrain channel IFL
High-pec Not Detected primordial hindbrain channel angiogenic sprout IFL
adgra2s984/s984; s896Tg; ubs1Tg standard conditions Prim-15 cranial vasculature IFL
Prim-15 mid cerebral vein endothelial cell IFL
Prim-15 primordial hindbrain channel endothelial cell IFL
is4Tg; s982Tg standard conditions Prim-15 endothelial cell filamentous actin IFL
Prim-15 primordial hindbrain channel filamentous actin IFL
s843Tg standard conditions Prim-15 brain vasculature IFL
Prim-15 mid cerebral vein IFL
Prim-15 primordial hindbrain channel IFL
Prim-15 primordial hindbrain channel angiogenic sprout IFL
Prim-25 brain vasculature IFL
Prim-25 mid cerebral vein IFL
Prim-25 primordial hindbrain channel IFL
Prim-25 primordial hindbrain channel angiogenic sprout IFL
High-pec brain vasculature IFL
High-pec mid cerebral vein IFL
High-pec primordial hindbrain channel IFL
High-pec primordial hindbrain channel angiogenic sprout IFL
s896Tg; ubs1Tg standard conditions Prim-15 cranial vasculature IFL
Prim-15 mid cerebral vein endothelial cell IFL
Prim-15 primordial hindbrain channel endothelial cell IFL
mCherry adgra2s984/s984; is4Tg; s982Tg standard conditions Prim-15 mid cerebral vein endothelial cell IFL
Prim-15 primordial hindbrain channel endothelial cell IFL
adgra2s984/s984; s1010Tg; s896Tg standard conditions Prim-25 basilar artery IFL
Prim-25 cranial vasculature IFL
Prim-25 posterior communicating artery IFL
Prim-25 primordial hindbrain channel IFL
adgra2s984/s984; s896Tg; ubs1Tg standard conditions Prim-15 cranial vasculature IFL
Prim-15 mid cerebral vein endothelial cell IFL
Prim-15 primordial hindbrain channel endothelial cell IFL
is4Tg; s982Tg standard conditions Prim-15 mid cerebral vein endothelial cell IFL
Prim-15 primordial hindbrain channel endothelial cell IFL
s1010Tg; s896Tg standard conditions Prim-25 basilar artery IFL
Prim-25 brain vasculature IFL
Prim-25 cranial vasculature IFL
Prim-25 posterior communicating artery IFL
Prim-25 primordial hindbrain channel IFL
s896Tg; ubs1Tg standard conditions Prim-15 cranial vasculature IFL
Prim-15 mid cerebral vein endothelial cell IFL
Prim-15 primordial hindbrain channel endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
adgra2s984/s984; is4Tg; s982Tg standard conditions Prim-15 endothelial cell filamentous actin decreased accumulation primordial hindbrain channel dorsal region, abnormal
adgra2s984/s984; s843Tg standard conditions Prim-15 - High-pec primordial hindbrain channel angiogenic sprout absent, abnormal
Prim-15 - High-pec primordial hindbrain channel endothelial tip cell fate specification decreased process quality, abnormal
Prim-15 - High-pec primordial hindbrain channel sprouting angiogenesis decreased process quality, abnormal
adgra2s984/s984; s896Tg; ubs1Tg standard conditions Prim-15 primordial hindbrain channel endothelial cell amount, normal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife