FIGURE

Fig. 3

ID

ZDB-FIG-150721-2

Publication

Shin et al., 2014 - Efficient homologous recombination-mediated genome engineering in zebrafish using TALE nucleases

Other Figures

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Fig. 3

Germline transmission rates of sox2-2a-sfGFP knock-in lines. (A) The frequency of germline transmission of the founders that were co-injected with sox2 TALEN and each targeting construct, which was linearized by either NotI or NcoI. The bars represent the percentage of F0 founders that produced sfGFP-positive F1 progeny. (B,C) Comparison of sfGFP expression between strong and weak sox2-2a-sfGFP F1 embryos. Epifluorescence stereomicroscopic images of sfGFP expression. Lateral views of 1dpf embryos. Both images were taken with the same exposure time. (B) The strong sfGFP-positive F1 embryos obtained from line 3. (C) The weak sfGFP-positive F1 embryos obtained from line 4. Scale bar: 200µm.

Expression Data

Gene:	GFP
Fish:	stl84Tg (AB)
Anatomical Term:	brain
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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