ZFIN ID: ZDB-FIG-150624-4
Wolman et al., 2015 - A Genome-wide Screen Identifies PAPP-AA-Mediated IGFR Signaling as a Novel Regulator of Habituation Learning. Neuron   85(6):1200-11 Full text @ Neuron
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

Genetic Screen Identifies Mutations Affecting Acoustic Startle Habituation

(A) Schematic of acoustic startle habituation assay. Larvae are exposed to 10 non-habituating acoustic stimuli, delivered at 20 s interstimulus intervals (ISI), and then 30 habituating stimuli at a 1 s ISI.

(B) Mean acoustic startle habituation percentage calculated by comparing the average frequency of startle responsiveness of an individual to stimuli 1-10 and stimuli 31-40 (Wolman et al., 2011). Behaviorally defined wild-type siblings shown in white bars, mutants in gray bars.

(C) Estimated truncated PCXAp171 protein in information overloadp171 mutants due to Y1163X mutation. BC, biotin carboxylase; CT, carboxyl transferase; PT, pyruvate carboxylase tetramerization; BCCP, biotin-carboxy carrier protein.

(D) Estimated truncated PAPP-AAp170 protein product in unfilteredp170 mutants due to Q322X mutation. LG, laminin G-like module; LNR, Lin-12/Notch repeats, MPD, metzincin proteolytic domain containing zinc-binding consensus sequence (Zn) and Met-turn motif (M); CCP, complement control protein modules 1-5.

E) pappaap170 larvae injected with increasing doses of wild-type pappaa mRNA show improved habituation at 5 dpf. *p < 0.01, **p < 0.001, ANOVA with Bonferonni correction versus wild-type sibling (B) or uninjected pappaap170 (E) larvae. N = number of larvae shown within or below each bar. Error bars indicate SEM.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ap2s1p172/p172 standard conditions Day 5 habituation decreased process quality, abnormal
fgtp182/p182 standard conditions Day 5 habituation decreased process quality, abnormal
fgtp183/p183 standard conditions Day 5 habituation decreased process quality, abnormal
kcna1ap181/p181 standard conditions Day 5 habituation decreased process quality, abnormal
pappaap170/p170 standard conditions Day 5 habituation decreased process quality, abnormal
pcxap171/p171 standard conditions Day 5 habituation decreased process quality, abnormal
unm_p173p173/p173 standard conditions Day 5 habituation decreased process quality, abnormal
unm_p175p175/p175 standard conditions Day 5 habituation decreased process quality, abnormal
unm_p176p176/p176 standard conditions Day 5 habituation decreased process quality, abnormal
unm_p177p177/p177 standard conditions Day 5 habituation decreased process quality, abnormal
unm_p178p178/p178 standard conditions Day 5 habituation decreased process quality, abnormal
unm_p179p179/p179 standard conditions Day 5 habituation decreased process quality, abnormal
unm_p180p180/p180 standard conditions Day 5 habituation decreased process quality, abnormal
zdhhc17p174/p174 standard conditions Day 5 habituation decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neuron for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neuron