ZFIN ID: ZDB-FIG-150618-14
Dow et al., 2015 - Cellular projections from sensory hair cells form polarity-specific scaffolds during synaptogenesis. Genes & Development   29:1087-94 Full text @ Genes & Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 3 Afferent neurons traverse projections that extend to mature synapses. (A) An SBEM reconstruction shows a hair cell projection (green) extending into an aggregation of variously colored axonal terminals beneath mature ribbon synapses made by another hair cell (yellow). (B) In a frame from a time-lapse sequence, a projection (arrowhead) extends toward a presumptive ribbon synapse (asterisk). (C) A series of time-lapse confocal images shows extension of an afferent terminal along a projection (arrowheads). Times are denoted in minutes after rearrangement. (D) A reconstructed afferent terminal (magenta) traverses a hair cell projection (cyan). (E) An SBEM reconstruction demonstrates that all neurons contact the nascent cell soma on its two projections (asterisks). (F) Projections arising from anteriorly and posteriorly polarized hair cells make the greatest areas of contact with afferent neurons of the same polarity. P < 0.02; N = 4. (G) An unusually large projection (arrowhead) forms in the absence of lateral line neurons. (H) The reconstructed sibling hair cells from a larva whose lateral line ganglion had been ablated exhibit numerous and lengthy projections. (I) In the absence of lateral line neurons, projections show reduced stability. P < 0.01; N = 12 and 11.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP c264Tg; nkhsp70GFF4AEt; vo8Tg standard conditions Protruding-mouth neuromast hair cell cell body IFL
Protruding-mouth neuromast hair cell cell projection IFL
c264Tg; nkhsp70GFF4AEt; vo8Tg light ablation: posterior lateral line ganglion Protruding-mouth neuromast hair cell cell body IFL
Protruding-mouth neuromast hair cell cell projection IFL
mCherry c264Tg; nkhsp70GFF4AEt; vo8Tg standard conditions Protruding-mouth posterior lateral line axon terminus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
c264Tg; nkhsp70GFF4AEt; vo8Tg standard conditions Protruding-mouth neuromast hair cell cell projection present, normal
c264Tg; nkhsp70GFF4AEt; vo8Tg light ablation: posterior lateral line ganglion Protruding-mouth neuromast hair cell cell projection increased size, abnormal
Protruding-mouth neuromast hair cell cell projection transient, abnormal
Protruding-mouth neuromast hair cell ribbon synapse present, normal
Protruding-mouth whole organism lacks all parts of type posterior lateral line ganglion neuron projection terminus, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genes & Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genes & Dev.