FIGURE

Fig. 3

ID

ZDB-FIG-150514-1

Publication

Hou et al., 2014 - Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents

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Fig. 3

Figure 3. Mapping simultaneous V_m and Ca²⁺ in the zebrafish heart in vivo. (A) Fluorescence of GCaMP5G in a fish expressing CaViar under the cmlc2 promoter (Supplementary Movie 1). (B) Single optical section of a zebrafish heart expressing CaViar at 4 dpf (Supplementary Movie 2). Cell membranes fluoresced in both the voltage channel (top) and the Ca²⁺ channel (bottom) as the AP propagated from the atrium to the ventricle. (C) Map of AP isochrones overlaid on a three-dimensional reconstruction of the heart. AP onsets were measured as time to reach 5% of maximum on the rising edge, with sub-frame timing achieved through spline interpolation. (D) Three-dimensional reconstruction of the electrical AP (Supplementary Movie 3). Scale bars in (A–D) 50 μm.

Expression Data

Gene:	GCaMP
Fish:	a300Tg (AB)
Anatomical Term:	heart
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.