Fig. 4

Ciliary defects in zebrafish ift46 morphants. Immunofluorescence with antibody against acetylated α-tubulin showing cilia in the pronephric ducts, (scale bar, 10 µm) (A–A′), otic vesicle, (scale bar, 10 µm) (B–B′), olfactory placode, (scale bar, 10 µm) (C–C′) and a lateral line hair cell, (scale bar, 5 µm) (D–D′). The ift46 morphants exhibit shortened or fewer cilia compared with the control fish in all of these organs. Arrow head: tether cilia. Arrow: short cilia. (E–F′) SEM images showing the cilia in the olfactory placode of control fish (E, E′) and ift46 morphants (F, F′) at 5 dpf. Scale bar, 40 µm (E) and (F), 4 µm (E′) and (F′) (G–H′) TEM images of cilia in zebrafish pronephric ducts at 72 hpf. Ultrastructure of normal pronephric cilia shows 9+2 microtubule architecture at 72 hpf which is intact in ift46 morphants. (G′, H′) Representative views of individual cilium at higher magnification. Scale bar, 5 µm (G) and (H), 100 µm (G′) and (H′).