Fig. 6

Periostin localization patterns in zebrafish and medaka injured hearts. A–J: Zebrafish hearts immunostained with anti-zebrafish periostin antibodies. K–T: Medaka hearts immunostained with anti-medaka periostin antibodies. A–E: Zebrafish periostin-a localization patterns. Periostin-a was localized at atrio-ventricular (av) valves and ventriculo-bulbal (vb) valves in the uncut heart (A). No localization was observed in the wound after resection (B–E). F–J: Zebrafish periostin-b localization patterns. Periostin-b was localized in the epicardium and valves (data not shown) in the uncut heart (F). The localization was observed at the wound site at 7 dpa (G), 14 dpa (H), and 21 dpa (I). Endocardial localization was detected at 14 dpa (H, arrowhead) and 21 dpa (I, arrowhead). The localization was restricted to the epicardium by 30 dpa (J, arrowhead). K–O:. Medaka periostin-a localization patterns. Periostin-a localization was observed in the epicardium and valves in the uncut heart (K). The localization was observed in the epicardium during repair (L–O), and its expression seemed to cover the wound. The signal was also observed in the endocardium occasionally (N, white arrow). P–T: Medaka periostin-b localization patterns. Periostin-b was localized in the atrium (P) and valves (data not shown). The localization was observed at the wound and the injury border from 7 dpa to 30 dpa (Q–T) Z postn, zebrafish periostin; M postn, medaka periostin. Arrowheads indicate periostin localization. The dashed line indicates the approximate injury border. at, atrium; av, atrio-ventricular valves; ba, bulbus arteriosus; epi, epicardium; myo, myocardium; v, ventricle; vb, ventriculo-bulbal valves; wo, wound area. Shown are representative images from experiments repeated > 3 times. Scale bars = 100 µm.