ZFIN ID: ZDB-FIG-150420-5
Paolini et al., 2015 - Asymmetric inheritance of the apical domain and self-renewal of retinal ganglion cell progenitors depend on Anillin function. Development (Cambridge, England)   142(5):832-9 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 1 Anillin expression during zebrafish retinal differentiation. (A) anillin mRNA (blue) at 28 hpf. (B) Expression of the anillin-eGFP transgene. R, retina; T, optic tectum; L, lens. Left, z-projection view; middle and right, z-plane. (C,C2) anillin:anillin-eGFP/ath5:gap43-RFP transgenic retina. anillin-eGFP is downregulated in the differentiating cells of the RGC layer (asterisks). (D) Anillin-GFP localisation in the nucleus (asterisk), cleavage furrow (arrow, t=02) and at the midbody (arrow, t=52 and t=10′). Time, minutes. (E,E′) Anillin-eGFP symmetric (E) and asymmetric (E′) distribution and intensity profiles (data points and fitted Gaussian) used to detect the offset of the Anillin-eGFP spot from the daughter cell boundary. (F) The position of apical Anillin-eGFP (n=15 divisions). Distance from the cell boundary within the average radius of the Anillin spot (grey) is used to define symmetry (see supplementary material Fig. S3). (G) Frames from supplementary material Movie 1. The Anillin-eGFP spot-inheriting daughter (white arrowhead, red dot) migrates to the RGC layer. The sibling (blue dot) migrates back to the apical surface (n=5 out of 5 analysed divisions). At t=20′ a rotated frontal z-section (dotted lines, 3D slicing mode) across the centre of the dividing daughters is shown (oriented along the z-plane). The apical surface of the retinal neuroepithelium is to the top. Scale bars: 110µm in B; 25µm in C,C′; 6µm in E,E′; 12µm in G.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
anln AB control Prim-5 brain ISH
Prim-5 optic cup ISH
Prim-5 optic tectum ISH
EGFP cu2Tg; uh4Tg control Prim-5 optic tectum IFL
Prim-5 to Long-pec retina IFL
Prim-5 to Long-pec retinal ganglion cell cleavage furrow IFL
Prim-5 to Long-pec retinal ganglion cell midbody IFL
Prim-5 to Long-pec retinal ganglion cell nucleus IFL
Prim-15 retina IFL
uh4Tg(AB) control Prim-5 brain IFL
Prim-5 optic cup IFL
Prim-5 optic tectum IFL
RFP cu2Tg; uh4Tg control Prim-5 retinal ganglion cell IFL
Prim-5 to Long-pec retinal ganglion cell IFL
Prim-15 retinal ganglion cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development