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ZIRC
ZFIN ID: ZDB-FIG-150409-22
Keßler et al., 2015 - Loss of dihydrolipoyl succinyltransferase (DLST) leads to reduced resting heart rate in the zebrafish. Basic Research in Cardiology   110:468 Full text @ Basic Res. Cardiol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 4

Blockade of the citric acid cycle phenocopies the ste−/− mutant phenotype. ac, g Morpholino-modified antisense oligonucleotide mediated knockdown of OGDH subunit E1 (MO-E1) (a) or DLDH subunit E3 (MO-E3) (b) of the citric acid cycle phenocopies the ste−/− bradycardia (g), whereas injection of a control Morpholino has no effect (c, g). dg Incubation of wild-type embryos with the citric acid cycle blocker sodiumfluoroacetate (SFA) and the respiratory chain uncoupler 2,4-dinitrophenol (DNP) phenocopies the heart rate defect of ste−/− mutant embryos in a dose-dependent manner

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: 2,4-dinitrophenol Protruding-mouth heart contraction decreased rate, abnormal
Protruding-mouth pericardium edematous, abnormal
WT chemical treatment: sodium fluoroacetate Protruding-mouth heart contraction decreased rate, abnormal
Protruding-mouth pericardium edematous, abnormal
WT + MO1-dldh standard conditions Protruding-mouth heart contraction decreased rate, abnormal
Protruding-mouth pericardium edematous, abnormal
WT + MO1-ogdha + MO1-ogdhb standard conditions Protruding-mouth heart contraction decreased rate, abnormal
Protruding-mouth pericardium edematous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Basic Research in Cardiology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Basic Res. Cardiol.