Fig. 2

Examples of images representing all the analyzed phenotypes.

“Without phenotype” defect, also called “Normal” embryos (A) are without any phenotype. The “Dead” phenotype (B) is shown as totally necrosed, while the “Chorion” phenotype (C) represents embryos that are still located in their chorion. In “Down Curved Tail” (D), the tail is obviously oriented downward compared to the horizontal. “Hemostasis” (E) presents a small amount of blood which can be located everywhere in the embryo (mainly in the head or in the pericardial area). “Necrosed Yolk Sac” (F) corresponds to a darker yolk compared to normal. In the “Edema” phenotype (G), an edema generally surrounds the anteroventral part of the fish. The “Short Tail” phenotype (H) describes a tail shorter than normal. “Up Curved Fish” (I) and “Up Curved Tail” (J) are two slightly different phenotypes. There is a curvature on the back of the embryo for “Up Curved Fish” (like a kind of lordosis), whereas the curvature is located on the tail for the “Up Curved Tail” phenotype.