FIGURE

Fig. 1

ID

ZDB-FIG-150401-1

Publication

Fieramonti et al., 2015 - Quantitative measurement of blood velocity in zebrafish with optical vector field tomography

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Fig. 1

(a) Outline of the experimental setup. The light emitted from a white LED is projected on the sample (S) by a telecentric lens (TL). A telecentric objective (TO) creates the bright-field image of the sample on a CMOS camera. The sample is rotated along the vertical axis by a rotation stage (not shown). (b) Picture of a 5 dpf zebrafish. The acquisition region is indicated by the black rectangle. (c) 3D Reconstruction of the notochord and somites of the zebrafish (grey) obtained with OPT superimposed with the vasculature (red) obtained with Flow-OPT. Scale bar is 100 µm. (d–e) Close-up of the vasculature obtained with Flow- OPT (d) and SPIM (e). Flow-OPT visualizes only the patent vessels. Labeled vessels are: Caudal Vein (CV), Caudal Artery (CA), Vertebral Arteries (VTAs) and Dorsal Longitudinal Anastomotic Vessels (DLAVs).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biophotonics