ZFIN ID: ZDB-FIG-150206-12
Benard et al., 2014 - Phagocytosis of mycobacteria by zebrafish macrophages is dependent on the scavenger receptor Marco, a key control factor of pro-inflammatory signalling. Developmental and comparative immunology   47(2):223-33 Full text @ Dev. Comp. Immunol.
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 3

Morphants of marco are capable of phagocytosing dead cells. Phagocytosis of dying cells by macrophages was monitored in time. marco morphants and their controls were imaged after metronidazole treatment which induces nitroreductase-mediated cell ablation in a subpopulation of macrophages (mCherry positive cells). All macrophages express GFP. Time points represented are (A) time of nitroreductase-positive cells undergoing cell death, (B) phagocytosis of the dying cells by healthy GFP-positive macrophages and (C) migration of the macrophage containing the dead cell. Time lapse recordings were made of 4 embryos per group and examples of phagocytosis are shown for 2 control embryos en 2 marco morphants. Quantification of phagocytosis events are shown in Supplementary Fig. S2B. Asterisks indicate the phagocytosing macrophage; mpt: minutes post treatment.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gl22Tg; gl24Tg; i149Tg + MO1-marco bacterial treatment: Mycobacterium marinum Prim-15 apoptotic cell clearance process quality, normal
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Comp. Immunol.