FIGURE

Fig. 5

ID

ZDB-FIG-150120-23

Publication

Sheets et al., 2014 - Characterization of Ribeye Subunits in Zebrafish Hair Cells Reveals That Exogenous Ribeye B-Domain and CtBP1 Localize to the Basal Ends of Synaptic Ribbons

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Fig. 5

CtBP1 localizes to synaptic ribbons, but does not disrupt endogenous Ribeye.

Representative images of immunolabel in posterior lateral line NM3 hair cells of 5 dpf larvae. Scale bars: 3 μm (main panels), 1 μm (insets). (A) Ribeye b antibody labeling of synaptic ribbons (magenta) and MAGUK antibody labeling of postsynaptic densities (yellow) in a WT sibling larva. Anti-myc (cyan) immunolabel was performed as a negative control. (B–C) CtBP1-myc (cyan), Ribeye b (magenta), and MAGUK (yellow) immunolabel in two representative transgenic larvae. (B) CtBP1-myc (cyan) is strongly localized to the nucleus with weak synaptic localization. The red arrow indicates a synaptic ribbon containing CtBP1-myc. Note that Ribeye immunolabel intensity in all four synaptic ribbons appears comparable. (C) CtBP1-myc (cyan) is weakly localized to the nucleus with strong synaptic localization. Presynaptic Ribeye immunolabel intensity is not reduced compared to WT (A).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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