ZFIN ID: ZDB-FIG-141112-14
Schindler et al., 2014 - Hand2 elevates cardiomyocyte production during zebrafish heart development and regeneration. Development (Cambridge, England)   141(16):3112-22 Full text @ Development
Anatomical Term:
Stage: Prim-25

Fig. 5

Increased proliferation contributes to cardiac expansion in hand2-overexpressing embryos. (A-F) EdU incorporation in hearts of (A-C) nontransgenic and (D-F) Tg(hsp70:hand2) embryos at 36hpf, following heat shock at 10hpf and EdU pulse at 17hpf. Partial reconstructions of confocal z-stacks with ventricle upwards (A,C,D,F) and representative single slices (B,E). (A,D) White dots indicate EdU-positive (red) cells that are also MF20-positive (green) differentiated cardiomyocytes. (B,E) Arrows indicate EdU-positive cells that are also MF20 positive; DAPI (blue) marks all nuclei. (C,F) White dots indicate all myocardial nuclei. Qualitative assessment suggests increased numbers of EdU-positive cardiomyocytes in hand2-overexpressing hearts (D,E), particularly in the distal region of the ventricle and the outflow tract. (G) Timeline of experimental design. (H) Proliferation indices in nontransgenic (mCherry-negative) and Tg(hsp70:hand2) (mCherry-positive) embryos; error bars indicate s.d. Proliferation index was calculated by dividing the number of EdU-positive cardiomyocytes by the total number of cardiomyocytes. A significant increase in proliferation index was evident in hand2-overexpressing embryos (n=8 or 9; *P<0.001).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT control Prim-25 presumptive cardiac ventricle heart tube
IHC sd28Tg heat shock Prim-25 presumptive cardiac ventricle heart tube
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development