Fig. 7

nlk MO affects expression of MHB and diencephalon markers. Embryos were injected with nlk or wnt8b MOs. At bud stage, embryos were stained with either a mix of opl, pax2 and tbx6 (A,B,C; dorsal view, anterior to top), or with en2 (D,E,F; dorsal view, anterior to left). At 7 somites, embryos were stained with either en2 (G,H,I; dorsal view, anterior to left) or pax6 (J,K,L; dorsal view, anterior to top). Although expression of opl and pax2 are unaffected in wnt8b morphants (B) compared with wild type (A), nlk morphants have a slight reduction in pax2 expression (C; arrow). en2 expression is reduced in wnt8b MO (E), and is eliminated in nlk MO (F) compared with wild type (D). At 7 somites, en2 is expressed in the MHB and midbrain (G). Expression is substantially reduced in both wnt8b and nlk morphants (H,I; brackets delineate expression domain). pax6 expression is unchanged in wnt8b MO (K), but is reduced slightly in nlk MO (L; brackets indicate AP extent of expression domain).