ZFIN ID: ZDB-FIG-140624-23
Samson et al., 2013 - 3-OST-7 regulates BMP-dependent cardiac contraction. PLoS Biology   11(12):e1001727 Full text @ PLoS Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 2

Noncontracting ventricle in 3-OST-7 morphants generates normal action potentials and calcium transients.

(A) Zebrafish embryos were injected with 3-OST-7 MO1 at one-cell stage and allowed to develop until 48 hpf. The heart was then dissected out of the embryo and either placed in a recording chamber perfused with external control solution (for action potential analysis) or incubated in Fluo-4 and imaged using a confocal microscope (for calcium transient analysis). Control 48 hpf hearts from uninjected embryos or injected with control 3-OST-5 MO were processed in parallel. (B) A suction pipette was used to patch clamp the heart at the atrial or ventricular region. Action potentials were recorded from atria (C–D) and ventricles (E–F) of control (uninjected, WT) (C and E) and 3-OST-7 morphant (D and F) embryos. The action potentials were comparable between control and 3-OST-7 morphant embryos. Regions were selected from atrium and ventricle to record the calcium transients (G–J) and measure the Ca2+ transient amplitude (K) and Ca2+ transient decay (L) (error bars, SEM). No significant difference was detected between control and 3-OST-7 morphant embryos (p = 0.30 for atrial transient amplitude; p = 0.16 for ventricular transient amplitude; p = 0.37 for atrial transient decay; and p = 0.51 for ventricular transient decay). In the second technique to image the calcium transients, 3-OST-7 MO1 or control 3-OST-3Z MO was injected into Tg(cmlc2:gCaMP)s878 embryos at one-cell stage and allowed to develop until 48 hpf. Optical maps of calcium activation for a single cycle, represented by isochronal lines every 20 ms, show calcium activation and conduction velocity proceed normally in 3-OST-7 morphants (N) compared to control MO injected embryos (M). Conduction proceeds from the atrium through the AV canal to the ventricle and numbers indicate the temporal sequence of calcium activation. Color bar chart fluorescence intensity changes on a scale of 0 to 100. b.a., bulbus arteriosus.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-hs3st1l1 standard conditions Long-pec atrial cardiac muscle cell action potential process quality, normal
Long-pec regulation of cardiac muscle contraction by calcium ion signaling process quality, normal
Long-pec ventricular cardiac muscle cell action potential process quality, normal
s878Tg + MO1-hs3st1l1 standard conditions Long-pec regulation of cardiac muscle contraction by calcium ion signaling process quality, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.