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ZFIN ID: ZDB-FIG-140522-30
Ahuja et al., 2014 - Kappe neurons, a novel population of olfactory sensory neurons. Scientific Reports   4:4037 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 3

Go-ir-positive neurons do not co-localize with established markers for ciliated and microvillous neurons. (a-d), Double labeling of Go-ir-positive cells with different markers is analysed in horizontal cryostat sections of olfactory epithelia; dashed line, basal border; dotted line, apical border of the sensory layer; scale bar, 20µm. (a) Double fluorescent labeling of anti-Go antibody (green) with RFP (red) expressed in ciliated neurons in Tg(OMP:lyn-mRFP) shows absence of co-localization; filled grey arrowhead, Go-ir-positive cell; open arrowhead, RFP-positive cell. (b) Double fluorescent labeling of anti-Go antibody with Venus expressed in microvillous neurons in Tg(TRPC2:Venus) line shows absence of co-localization. Go-ir signal is set to green, Venus signal is set to red; filled arrowhead, Go -ir-positive cell; open arrowhead, Venus-positive cell. (c) Double fluorescent labeling of anti- Go antibody (green) with in situ hybridisation signal from TRPC2 probe5 shows absence of co-localization; filled grey arrowhead, Go-ir-positive cell; open arrowhead, TRPC2-positive cell. (d) Double fluorescent labeling of anti-Go antibody (green) with anti-calretinin antibody (red) shows absence of co-localization; filled grey arrowhead, Go-ir-positive cell; open arrowhead, calretinin-positive cell. (e) The empirical cumulative distribution function (ECDF) for a cell shape parameter (diameter ratio) shows distributions for TRPC2 and OMP-positive cells to be different from each other as well as from Go-ir and TrkA-ir, shown for comparison here. (f) Quantification of co-label for Go-ir and markers for microvillous, ciliated and crypt neurons (as indicated) shows 0 to 2% co-label (yellow) in Go-ir-positive neurons. Such small percentages amount to a handful of cells in an entire olfactory epithelium, and are likely to accrue from the dense packing of cells, dendrites, cilia and microvilli, at the limit of light-microscopic resolution. (g) The empirical cumulative distribution function (ECDF) for a cell localisation parameter (laminar height) shows distributions for TRPC2 and OMP-positive cells to be different from each other as well as from Go-ir and TrkA-ir-positive cells, shown for comparison here. (h) Maximal vertical distance (Δ max) of distributions as indicated; ø ratio, diameter ratio; height, normalized laminar height. Significance of distribution differences is assessed by Kolmogorov-Smirnov test of the unbinned distributions; a, p < 10-6; b, p > 0.6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
RFP rw035Tg standard conditions Adult ciliated olfactory receptor neuron IFL
trpc2b WT standard conditions Adult microvillous olfactory receptor neuron ISH
Venus rw036Tg standard conditions Adult microvillous olfactory receptor neuron IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-gnao1 IHC WT standard conditions Adult Not Detected ciliated olfactory receptor neuron
IHC Adult olfactory rosette sensory neuron
IHC rw035Tg standard conditions Adult Not Detected ciliated olfactory receptor neuron
IHC Adult olfactory rosette sensory neuron
IHC rw036Tg standard conditions Adult Not Detected microvillous olfactory receptor neuron
IHC Adult olfactory rosette sensory neuron
Ab4-calb2 IHC WT standard conditions Adult ciliated olfactory receptor neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.