FIGURE

Fig. S1

ID

ZDB-FIG-140506-73

Publication

Stewart et al., 2014 - Sequential and opposing activities of Wnt and BMP coordinate zebrafish bone regeneration

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Fig. S1

Pre-existing osteoblasts generate Runx2+ blastema cells. (A-C) Whole-mount epifluorescence microscopy of a mosaic adult Tg(Xla.ef1a1-actb2:LOXPLOX5171- FRT-F3-EGFP,mCherry); Tg(dusp6:CreERT2,myl7:ECFP) caudal fin mosaic displaying osteoblasts labeled by permanent mCherry expression (magenta) before amputation (A) and at 24 hpa (B). (C) Immunostaining of a section of this fin ray at 24 hpa showing Runx2 (green) and mCherry expression (red) in cells distal to the amputation site (dashed yellow line). Nuclei stained with Hoechst are shown in blue. (D-F) Another Tg(Xla.ef1a1-actb2:LOXPLOX5171- FRT-F3-EGFP,mCherry); Tg(dusp6:CreERT2,myl7:ECFP) osteoblast mosaic visualized by whole-mount epifluorescence microscopy at 25x (D). The region in the dashed box is shown at 120x magnification (E). (F) Immunostaining of a section of this mosaic fin at 48 hpa showing Runx2 (green) and mCherry (red) co-expression. Nuclei are stained with Hoechst (blue). The dashed yellow line marks the amputation plane. Magenta arrows point to the osteoblasts expressing mCherry in mosaic lepidotrichia and yellow arrows indicate the Runx2+/mCherry+ mosaic-derived cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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