ZFIN ID: ZDB-FIG-140414-18
Johnson et al., 2014 - Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube. Developmental Biology   387(1):73-92 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: 10-13 somites to Protruding-mouth

Fig. 5 kif11 mutants have inversely proportional changes to the number of cells dying and proliferating. (A,B) Lateral view of the 25 hpf neural tube labeled for anti-activated Caspase-3 (green) and anti-Gfap (red) antibodies in wild type (A) and kif11-/- mutants (B). (C,D) Lateral view of the 25 hpf neural tube labeled for anti-BrdU (green) and anti-Gfap (red) antibodies in wild type (C) and kif11-/- (D). (E) Quantification of cell death (dashed lines) and BrdU incorporation (solid lines). Anti-activated Caspase-3 labeling was increased in kif11-/- mutants from 15 hpf to 72 hpf (E, dashed blue line), whereas the number of cells in S-phase was reduced from 20 hpf to 36 hpf in kif11-/- mutants (E, solid black line) as compared with wild type embryos (black lines). (F) Graphical representation of the difference between the number of cells dying (dashed line) or BrdU positive (solid line) between kif11 siblings.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zrf-1 IHC WT standard conditions Prim-5 radial glial cell
IHC kif11hi3112aTg/hi3112aTg standard conditions Prim-5 radial glial cell
Phenotype Details
Fish Conditions Stage Phenotype
kif11hi3112aTg/hi3112aTg standard conditions 10-13 somites - Protruding-mouth neural tube apoptotic process increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(1), Johnson, K., Moriarty, C., Tania, N., Ortman, A., Dipietrantonio, K., Edens, B., Eisenman, J., Ok, D., Krikorian, S., Barragan, J., Golé, C., and Barresi, M.J., Kif11 dependent cell cycle progression in radial glial cells is required for proper neurogenesis in the zebrafish neural tube, 73-92, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.