FIGURE

Fig. 3

ID

ZDB-FIG-140311-4

Publication

Becker et al., 2014 - Differential activation of natriuretic peptide receptors modulates cardiomyocyte proliferation during development

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Fig. 3

Manipulation of cardiac natriuretic peptide expression alters total cardiomyocyte numbers in the embryonic heart. (A) Total cardiomyocytes at 48 hpf were increased in the Nppa/Nppb double knockdown (nppa/nppb MO) embryos, but were decreased in the nppb overexpression embryos (HS/nppb). ^*P<0.02 compared with control. (B) Representative images from cmlc2:CFP (pseudo-colored green) and DAPI (pseudo-colored red) stained hearts. (C) The total number of apoptotic heart cells was not increased in the nppb overexpression embryos. P>0.05 compared with control. (D) Knockdown of Nppa/Nppb and overexpression of nppb were able to significantly alter cardiomyocyte proliferation at 52 hpf. ^*P<0.03 compared with control. (E) Representative images of BrdU-labeled (red) cmlc2:CFP (green)-positive heart tissue. White arrowhead indicates BrdU-positive cardiomyocytes. Intracavitary BrdU+ cells were considered to be endothelial in origin and were not counted. All data are expressed as mean + s.e.m. Scale bars: 100 μm.

Expression Data

Gene:	CFP
Fish:	bw1Tg; bw2Tg
Anatomical Term:	cardiac muscle cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bw1Tg; bw2Tg WT + MO1-nppb + MO2-nppa
Knockdown Reagents:	MO1-nppb MO2-nppa
Observed In:	cardiac muscle cell cardiac muscle cell apoptotic process cardiac muscle cell proliferation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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