ZFIN ID: ZDB-FIG-140124-29
Brothers et al., 2013 - NADPH Oxidase-Driven Phagocyte Recruitment Controls Candida albicans Filamentous Growth and Prevents Mortality. PLoS pathogens   9(10):e1003634 Full text @ PLoS Pathog.
Anatomical Terms:
Stage: Prim-25

Fig. 2

NADPH oxidase is required for efficient phagocyte recruitment and containment of C. albicans.

(A–F) CAF2-dTomato C. albicans or were microinjected into the hindbrain ventricle of (A) Tg(mpx:GFP)i114 (n = 11) or (B) Tg(mpeg1:GAL4/UAS:Kaede) (n = 15) prim25 stage larvae. Fish were treated with DMSO (vehicle) or DPI from two hours prior to infection to 4 hpi and imaged from 0 to 4 hpi by confocal microscopy. (A–B) Images represent at least three independent experiments with three fish of each group per experiment. Scale bars = 10 μm. (C,D) Tg(mpx:GFP)i114 fish with GFP-expressing neutrophils were used. (E–F) Tg(mpeg1:GAL4/UAS:Kaede) fish with Kaede-expressing macrophages were used. (C, E) Phagocytes were counted at the site of infection at 4 hpi. Total phagocytes includes all EGFP+ phagocytes (both with and without yeast) and EGFP phagocytes that internalized fungi. Phagocytes with Candida includes only phagocytes with internalized fungi. Data from at least three independent experiments was pooled and the average and standard error of all fish are shown. (D, F) At 4 hpi, fungi were scored as intracellular or extracellular, and the % internal was calculated per fish. Average and standard error are shown. *p<0.05 **p<0.01.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP i114Tg standard conditions Prim-25 neutrophil IFL
Prim-25 phagocyte IFL
Kaede gl24Tg; s1999tTg standard conditions Prim-25 macrophage IFL
Prim-25 phagocyte IFL
Antibody Labeling Details No data available
Phenotype Details No data available
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Pathog.