ZFIN ID: ZDB-FIG-140124-15
Matsuda et al., 2013 - Aldh1-expressing endocrine progenitor cells regulate secondary islet formation in larval zebrafish pancreas. PLoS One   8(9):e74350 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Days 14-20 to Days 21-29

Fig. 4

Cell proliferation among Aldh1- and 2F11-expressing cells.

EdU signals (white arrows) were detected in subsets 2F11pos cells at 15 (A), 20 (B) and 25 dpf (C). (D) Cells co-expressing Aldh1 and 2F11 (blue arrows) are not proliferative, but are localized in the adjacent to Aldh1-negative, EdUpos, 2F11pos cells (white arrows). (E and E′) Cells co-expressing NeuroD and 2F11 (yellow arrows) are not proliferative but are localized adjacent to NeuroD:eGFP-negative, EdUpos, 2F11pos cells (white arrows). (F and F′) Detailed geometry of 2F11, Aldh1, NeuroD:eGFP expression in association with EdU incorporation. White arrows indicate EdU pos/2F11 pos/Aldh1 neg/NeuroD:eGFPneg cells. Blue arrows indicate EdUneg /2F11 pos/Aldh1 pos/NeuroD:eGFPpos cells. Yellow arrows indicate EdU neg/2F11 pos/Aldh1 neg/NeuroD:eGFPpos cells. Green arrows indicate EdU neg/2F11neg /Aldh1 neg/NeuroD:eGFPpos cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP jh1Tg standard conditions Days 14-20 exocrine pancreas IHC
Days 21-29 exocrine pancreas IHC
nl1Tg standard conditions Days 14-20 pancreas endocrine cell IHC
Days 21-29 pancreas endocrine cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-2F11 IHC jh1Tg standard conditions Days 14-20 pancreatic duct epithelium
IHC Days 21-29 pancreatic duct epithelium
IHC nl1Tg standard conditions Days 14-20 pancreatic duct epithelium
IHC Days 21-29 pancreatic duct epithelium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One