ZFIN ID: ZDB-FIG-140117-39
Yogev et al., 2013 - eIF4EBP3L acts as a gatekeeper of TORC1 in activity-dependent muscle growth by specifically regulating Mef2ca translational initiation. PLoS Biology   11(10):e1001679 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 2

Mef2ca is up-regulated by muscle activity and essential for muscle growth.

(A–C) MS222 preferentially suppresses the level of Mef2c protein. Zebrafish embryos were incubated from 31–48 hpf with MS222 and Mef2 and MyoD (A, B) or specifically Mef2c (C) were detected by Western blot (50 embryos/sample). Whereas Mef2 decreased, Myod and Vinculin were unchanged relative to Actin control (B). (D–H) Fibers in the midbody region of wild-type embryos (D) or from a mef2catn213/+ incross (E, F) exposed to MS222 or vehicle for 17 h were immunostained for Mef2c (green) and Hoechst (D) and slow MyHC (E, G) or all MyHC (F, H). Mef2c staining identified mutants at the expected frequency. Myofibril bundle width was determined from at least four fibers in somite 17 of nine embryos in each condition (as shown by white arrows in G). Somite volume was calculated by multiplying the length of the somite 17 by the cross-section area (as shown in red in H, n = 17 embryos). Scale bars = 50 μm. Bars represent SEM and samples were compared by t test. All experiments were repeated at least twice.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-A4.1025 IHC WT standard conditions Long-pec fast muscle cell
IHC Long-pec slow muscle cell
IHC mef2catn213/tn213(TU) standard conditions Long-pec fast muscle cell
IHC Long-pec slow muscle cell
Ab-F59 IHC WT standard conditions Long-pec slow muscle cell
Ab1-mef2 WB WT standard conditions Long-pec whole organism
WB WT chemical treatment: tricaine methanesulfonate Long-pec whole organism
Ab1-myod1 WB WT standard conditions Long-pec whole organism
WB WT chemical treatment: tricaine methanesulfonate Long-pec whole organism
Ab2-act WB WT standard conditions Long-pec whole organism
WB WT chemical treatment: tricaine methanesulfonate Long-pec whole organism
WB chrndsb13/sb13(TU) standard conditions Long-pec whole organism
Ab2-mef2c WB WT standard conditions Long-pec whole organism
WB WT chemical treatment: tricaine methanesulfonate Long-pec whole organism
WB chrndsb13/sb13(TU) standard conditions Long-pec whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: tricaine methanesulfonate Long-pec skeletal muscle cell myofibril decreased diameter, abnormal
WT chemical treatment: tricaine methanesulfonate Long-pec skeletal myofibril assembly decreased occurrence, abnormal
chrndsb13/sb13(TU) standard conditions Long-pec skeletal myofibril assembly decreased occurrence, abnormal
mef2catn213/tn213(TU) standard conditions Long-pec myotome decreased volume, abnormal
Long-pec skeletal muscle cell myofibril decreased diameter, abnormal
Long-pec skeletal myofibril assembly decreased occurrence, abnormal
mef2catn213/tn213(TU) chemical treatment: tricaine methanesulfonate Long-pec skeletal muscle cell myofibril decreased diameter, abnormal
Long-pec skeletal myofibril assembly decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.