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Fig. S1

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ZDB-FIG-130430-7

Publication

Zarelli et al., 2013 - Inhibition of neural crest formation by Kctd15 involves regulation of transcription factor AP-2

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Fig. S1

Functional interactions between AP-2 and Kctd15. (A) Expression of tfap2a in the neural crest (NC() region was inhibited by kctd15 overexpression. Zebrafish embryos were injected with 80 pg of kctd15 mRNA, collected at bud stage, and hybridized to tfap2a probe. Fifty control and 57 kctd15-injected embryos were scored. In the histogram, embryos with the appearance in A,a correspond to blue, whereas embryos as in A, b correspond to purple. (B) Kctd15 tagged with the Flag-ONE-Strep (FOS) epitope maintains biological activity. Zebrafish embryos were injected with increasing concentrations of kctd15-FOS mRNA. In situ hybridization with the NC marker foxD3 shows inhibition in injected embryos, similar to that seen with untagged Kctd15 (1).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA