ZFIN ID: ZDB-FIG-130425-19
Drerup et al., 2013 - JNK-Interacting Protein 3 Mediates the Retrograde Transport of Activated c-Jun N-Terminal Kinase and Lysosomes. PLoS Genetics   9(2):e1003303 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 3 pJNK levels were elevated in jip3nl7 axon terminals.

(A–H) Immunolabeling for active JNK (pJNK; red in merge; white in single channel) in proximal (NM1) and distal (NM3) neuromasts at 2 and 5 dpf. pJnk levels were elevated in all axon terminals in jip3nl7 mutants (A–I; arrowheads). (I,J) Mean fluorescent intensity (background subtracted; see Materials and Methods for details) of pJNK and total JNK (tJNK) labeling in NM1 axon terminals and the pLL ganglion (pLLg) at 5 dpf. (ANOVA, post-hoc contrasts; *-p<0.001). Scale bars = 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mapk8ip3nl7/nl7; nl1Tg standard conditions Long-pec posterior lateral line axon IHC
Long-pec posterior lateral line axon terminus IHC
Day 5 posterior lateral line axon IHC
Day 5 posterior lateral line axon terminus IHC
nl1Tg standard conditions Long-pec posterior lateral line axon IHC
Long-pec posterior lateral line axon terminus IHC
Day 5 posterior lateral line axon IHC
Day 5 posterior lateral line axon terminus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab15-mapk IHC mapk8ip3nl7/nl7; nl1Tg standard conditions Long-pec posterior lateral line axon
IHC Long-pec posterior lateral line axon terminus
IHC Day 5 posterior lateral line axon
IHC Day 5 posterior lateral line axon terminus
IHC nl1Tg standard conditions Long-pec posterior lateral line axon
IHC Long-pec posterior lateral line axon terminus
IHC Day 5 posterior lateral line axon
IHC Day 5 posterior lateral line axon terminus
Phenotype Details
Fish Conditions Stage Phenotype
mapk8ip3nl7/nl7; nl1Tg standard conditions Long-pec posterior lateral line axon terminus swollen, abnormal
Long-pec regulation of JNK cascade decreased process quality, abnormal
Day 5 posterior lateral line axon terminus swollen, abnormal
Day 5 regulation of JNK cascade decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.