ZFIN ID: ZDB-FIG-130425-19 |
Drerup et al., 2013
- JNK-Interacting Protein 3 Mediates the Retrograde Transport of Activated c-Jun N-Terminal Kinase and Lysosomes.
PLoS Genetics
9(2):e1003303
Full text @ PLoS Genet.
Fig. 3 pJNK levels were elevated in jip3nl7 axon terminals. (A–H) Immunolabeling for active JNK (pJNK; red in merge; white in single channel) in proximal (NM1) and distal (NM3) neuromasts at 2 and 5 dpf. pJnk levels were elevated in all axon terminals in jip3nl7 mutants (A–I; arrowheads). (I,J) Mean fluorescent intensity (background subtracted; see Materials and Methods for details) of pJNK and total JNK (tJNK) labeling in NM1 axon terminals and the pLL ganglion (pLLg) at 5 dpf. (ANOVA, post-hoc contrasts; *-p<0.001). Scale bars = 10 μm. |
Gene Expression Details
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
---|---|---|---|---|---|---|
EGFP | mapk8ip3nl7/nl7; nl1Tg | standard conditions | Long-pec | posterior lateral line axon | IHC | |
Long-pec | posterior lateral line axon terminus | IHC | ||||
Day 5 | posterior lateral line axon | IHC | ||||
Day 5 | posterior lateral line axon terminus | IHC | ||||
nl1Tg | standard conditions | Long-pec | posterior lateral line axon | IHC | ||
Long-pec | posterior lateral line axon terminus | IHC | ||||
Day 5 | posterior lateral line axon | IHC | ||||
Day 5 | posterior lateral line axon terminus | IHC |
Antibody Labeling Details
Antibody | Assay | Fish | Conditions | Stage | Anatomy |
---|---|---|---|---|---|
Ab15-mapk | IHC | mapk8ip3nl7/nl7; nl1Tg | standard conditions | Long-pec | posterior lateral line axon |
IHC | Long-pec | posterior lateral line axon terminus | |||
IHC | Day 5 | posterior lateral line axon | |||
IHC | Day 5 | posterior lateral line axon terminus | |||
IHC | nl1Tg | standard conditions | Long-pec | posterior lateral line axon | |
IHC | Long-pec | posterior lateral line axon terminus | |||
IHC | Day 5 | posterior lateral line axon | |||
IHC | Day 5 | posterior lateral line axon terminus |
Phenotype Details
Acknowledgments: | ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet. |
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