ZFIN Figure: Drerup et al., 2013, Fig. 3

ZFIN ID: ZDB-FIG-130425-19

ZFIN ID: ZDB-FIG-130425-19

Drerup et al., 2013 - JNK-Interacting Protein 3 Mediates the Retrograde Transport of Activated c-Jun N-Terminal Kinase and Lysosomes. PLoS Genetics 9(2):e1003303 Full text @ PLoS Genet.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Antibody:	Ab15-mapk
Fish:	nl1Tg mapk8ip3^nl7/nl7; nl1Tg
Anatomical Terms:	posterior lateral line axon posterior lateral line axon terminus
Stage Range:	Long-pec to Day 5

PHENOTYPE:

Fish:	mapk8ip3^nl7/nl7; nl1Tg
Observed In:	posterior lateral line axon terminus regulation of JNK cascade
Stage Range:	Long-pec to Day 5

Fig. 3 pJNK levels were elevated in jip3nl7 axon terminals.

(A–H) Immunolabeling for active JNK (pJNK; red in merge; white in single channel) in proximal (NM1) and distal (NM3) neuromasts at 2 and 5 dpf. pJnk levels were elevated in all axon terminals in jip3^nl7 mutants (A–I; arrowheads). (I,J) Mean fluorescent intensity (background subtracted; see Materials and Methods for details) of pJNK and total JNK (tJNK) labeling in NM1 axon terminals and the pLL ganglion (pLLg) at 5 dpf. (ANOVA, post-hoc contrasts; *-p<0.001). Scale bars = 10 μm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	mapk8ip3^nl7/nl7; nl1Tg	standard conditions	Long-pec	posterior lateral line axon	IHC
			Long-pec	posterior lateral line axon terminus	IHC
			Day 5	posterior lateral line axon	IHC
			Day 5	posterior lateral line axon terminus	IHC
	nl1Tg	standard conditions	Long-pec	posterior lateral line axon	IHC
			Long-pec	posterior lateral line axon terminus	IHC
			Day 5	posterior lateral line axon	IHC
			Day 5	posterior lateral line axon terminus	IHC

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab15-mapk	IHC	mapk8ip3^nl7/nl7; nl1Tg	standard conditions	Long-pec	posterior lateral line axon
	IHC			Long-pec	posterior lateral line axon terminus
	IHC			Day 5	posterior lateral line axon
	IHC			Day 5	posterior lateral line axon terminus
	IHC	nl1Tg	standard conditions	Long-pec	posterior lateral line axon
	IHC			Long-pec	posterior lateral line axon terminus
	IHC			Day 5	posterior lateral line axon
	IHC			Day 5	posterior lateral line axon terminus

Phenotype Details

Fish	Conditions	Stage	Phenotype
mapk8ip3^nl7/nl7; nl1Tg	standard conditions	Long-pec	posterior lateral line axon terminus swollen, abnormal
		Long-pec	regulation of JNK cascade decreased process quality, abnormal
		Day 5	posterior lateral line axon terminus swollen, abnormal
		Day 5	regulation of JNK cascade decreased process quality, abnormal

Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.